AIM: Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning.

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Presentation transcript:

AIM:

Genetic Engineering: changing the DNA of living organisms. 1. Inserting genes into other organisms 2. Selective Breeding 3. Cloning

Transgenic organism- genes from more than one species Example: GFP mice, silk goats

First, we must ISOLATE the gene Restriction enzymes: cut DNA at specific sequences of nucleotides EcoRI cuts at “sticky end”

Separating DNA Gel electrophoresis can be used to separate DNA sequences

Applications of Transgenic Organisms

Bacteria can produce insulin

Plasmid contains antibiotic resistance= genetic marker

2. Selective Breeding

3. Cloning

Summary: Explain the role of restriction enzymes and plasmids in genetic engineering How could genetic engineering be used to treat medical conditions?