1. PLANT BIOTECHNOLOGY

2. Biotechnology ? The application of biological organisms, systems or processes to manufacturing and service industries The application of biological organisms, systems or processes to manufacturing and service industries The integrated use of biochemistry, microbiology and engineering sciences in order to achieve technological (industrial) application capabilities of microorganism, cultured tissue cells and part thereof The integrated use of biochemistry, microbiology and engineering sciences in order to achieve technological (industrial) application capabilities of microorganism, cultured tissue cells and part thereof A technology using biological phenomena by copying and manufacturing various kinds of useful substance A technology using biological phenomena by copying and manufacturing various kinds of useful substance The application of scientific and engineering principles to the processing of materials by biological-agents to provide goods and services The application of scientific and engineering principles to the processing of materials by biological-agents to provide goods and services

3. Biotechnology ? The science of the production processes based on the action of microorganisms and their active components and production processes involving the use of cells and tissues from higher organisms. It was not included medical technology, agriculture and traditional crop breeding The science of the production processes based on the action of microorganisms and their active components and production processes involving the use of cells and tissues from higher organisms. It was not included medical technology, agriculture and traditional crop breeding The use of living organism and their component in agriculture, food and other industrial processes The use of living organism and their component in agriculture, food and other industrial processes The use of microbial, animal and plant cells or enzymes to synthesize, breakdown and transform materials The use of microbial, animal and plant cells or enzymes to synthesize, breakdown and transform materials The integration of natural sciences and organisms, cells, parts thereof and molecular analogues to product and services The integration of natural sciences and organisms, cells, parts thereof and molecular analogues to product and services

4. Stages of Biotechnology Development Ancient biotechnology Ancient biotechnology early history as related to food and shelter; Includes domestication Classical biotechnology Classical biotechnology built on ancient biotechnology; Fermentation promoted food production, and medicine Modern biotechnology Modern biotechnology manipulates genetic information in organism; Genetic engineering

5.  Traditional/old biotechnology The conventional techniques that have been used to produce beer, wine, cheese, many other food  New/modern biotechnology All methods of genetic modification by recombinant DNA and cell fusion techniques, together with the modern development of traditional biotechnological process Stages of Biotechnology Development

6. Areas of Biotechnology Areas of Biotechnology Organismic biotechnology Organismic biotechnology uses intact organisms; Does not alter genetic material Molecular biotechnology Molecular biotechnology alters genetic makeup to achieve specific goals Transgenic organism- an organism with artificially altered genetic materialTransgenic organism- an organism with artificially altered genetic material

7. Biotechnology: A collection of technologies

8. The Applications of Biotechnology  Medical Biotechnology  Diagnostics  Therapeutics  Vaccines  Agricultural Biotechnology  Plant agriculture  Animal agriculture  Food processing  Environmental Biotechnology Cleaning through bioremediation Cleaning through bioremediation Preventing environmental problems Preventing environmental problems Monitoring the environment Monitoring the environment

9. Plant agriculture Crop production and protection Crop production and protection Genetically engineered (transgenic) cropsGenetically engineered (transgenic) crops Using biological methods to protect cropsUsing biological methods to protect crops Exploiting cooperative relationships in natureExploiting cooperative relationships in nature Nutritional value of crops Nutritional value of crops Improving food quality and safetyImproving food quality and safety Healthier cooking oils by decreasing the concentration of saturated fatty acids in vegetable oilsHealthier cooking oils by decreasing the concentration of saturated fatty acids in vegetable oils Functional foodsFunctional foods Foods containing significant levels of biologically active components that impart health benefits Plant Biotechnology

10. TRADITIONAL PLANT BIOTECHNOLOGY breeding tissue culture inter-specific hybridisation mapping phenotypic/biochemical markers

11. Now use a molecular approach to manipulation: molecular markers & mapping gene cloning plant transformation PLANT BIOTECHNOLOGY a process to produce a genetically modified plant by removing genetic information from an organism, manipulating it in the laboratory and then transferring it into a plant to change certain of its characteristics.

12. Exogenous genes (non-plant genes) Applications: transgenic pathogen-derived genes bacterial genes any other organism Pathogen resistance Herbicide resistance bioreactors Delivery systems

13. Endogenous genes (Plant genes ) Gene discovery (functional genomics) Applications: markers transgenic Enzymes in biochemical pathway Natural resistance genes Marker assisted breeding Plant improvement Mapping ESTs, libraries Silencing, expression Mutants, arrays

14. Outline I. Plant Tissue Culture and Applications A.Plant Tissue Culture B.Micro-propagation 1. Somatic Embryos 2. Chemicals from Plants C.Other Uses of Tissue Culture 1. Protoplast Fusion 2. Somaclonal Variation 3. Germplasm Storage II. Plant Genetic Engineering A.Plant Transformation B.Transgenic Plant

15. Outline III. Applications of Plant Genetic Engineering IV. Introduction to molecular marker V.A Bright Future

16. Grading system Grade : 0 – 100 Grade : 0 – 100 A> 80 A> 80 B – D → 45 – 80 (Normal distribution) B – D → 45 – 80 (Normal distribution) E < 45 E < 45 Grade composition Assignment:30 Mid-term:30 Final Exam :40