1. 9.4 Genetic Engineering Sponge 2 1. What is a DNA fingerprint? 2. Name a few ways that we can use DNA fingerprints.

2. 9.4 Genetic Engineering 1. What is a DNA fingerprint? 2. Name a few ways that we can use DNA fingerprints. A DNA fingerprint is a representation of parts of an individual’s DNA that can be used to identify a person at the molecular level. crime investigation- ID victim, ID criminal etc… Paternity- proving family relationships (Who da’ Dad???) Kinship necessary for immigration requests Used to study biodiversity Follow genetically engineered crops

3. 9.4 Genetic Engineering Click and Clone Questions 1.What two types of cells do you need in order to create a clone? 2.How many organisms does it take to make a clone? 3.Which two mice will be genetically identical? 4.Will the clone always look and act identical to its genetic donor? Please copy down the questions on p.110. Leave room for the answers.

4. 9.4 Genetic Engineering KEY CONCEPT DNA sequences of organisms can be changed. CC= Copy Cat or Carbon Copy

5. 9.4 Genetic Engineering Entire organisms can be cloned. A clone is a genetically identical copy of a gene or of an organism. CC- The first cloned cat Born Dec 22, 2001 after 86 unsuccessful tries

6. 9.4 Genetic Engineering Cloning occurs in nature. –bacteria (binary fission- makes a copy of itself) –some simple animals and plants (budding, regeneration) –Identical twins

7. 9.4 Genetic Engineering Mammals can be cloned through a process called nuclear transfer. –nucleus is removed from an egg cell –nucleus of a cell from the animal to be cloned is implanted in the egg

8. 9.4 Genetic Engineering Clone Mimi the Mouse http://learn.genetics.utah.edu/content/tech/cloning/clickandclone/

9. 9.4 Genetic Engineering Click and Clone Questions 1.What two types of cells do you need in order to create a clone? 2.How many organisms does it take to make a clone? 3.Which two mice will be genetically identical? 4.Will the clone always look and act identical to its genetic donor?

10. 9.4 Genetic Engineering Click and Clone Questions 1.What two types of cells do you need in order to create a clone? Somatic cell and egg cell 2. How many organisms does it take to make a clone? 3: Somatic cell donor (who we are cloning), egg donor, and surrogate (who carries the baby) 3. Which two mice will be genetically identical? Mimi and Mini Mimi (the clone) 4. Will the clone always look and act identical to its genetic donor? No. The behavior may be very different from the original.

11. 9.4 Genetic Engineering In 1997 Dolly became the first mammal to be cloned using an adult somatic (body) cell. She was born on the 277 th try. Dolly and her lamb Bonny MEET DOLLY She was derived from a mammary gland She was named after Dolly Parton Did you know?

12. 9.4 Genetic Engineering Carp (fish) Carp Cat Cattle Deer Dog Ferret Frog (tadpole) Frog Fruit Flies Gaur (wild cattle) Gaur Goat Horse Mice Mouflon (wild sheep) Mouflon Mule Pig Pyrenean ibex (type of goat) Pyrenean ibex *first extinct animal to be cloned- only lived 7 mins Rabbit Rat Rhesus Monkey Sheep Water Buffalo Wolf Known animals to have been cloned as of 2012:

13. 9.4 Genetic Engineering benefits –organs for transplant into humans –save endangered species concerns –low success rate (approx. 1-3 % are successful) –clones “imperfect” and less healthy than original animal –decreased biodiversity

14. 9.4 Genetic Engineering Issues with Dolly She developed and grew normally, but she had health problems Did not live as long as typical sheep

15. 9.4 Genetic Engineering Pet owners expecting to clone an exact copy of their furry friend will be disappointed… May not look, act, or behave like the original. Likely to have health issues, and a shorter life span. *CC does not have the health issues normally associated with other clones.

16. 9.4 Genetic Engineering Cloning in movies and books The Island (2005) People on the island are told what to eat, wear, and how to act. They are clones. Held prisoner to be used for spare parts or as surrogates The Boys from Brazil After WWII, Nazis create 94 Hitler clones in hopes that one will grow up to be like the original Hitler and create a fourth Reich

17. 9.4 Genetic Engineering The Clone Age When watching the video, please consider the advantages and disadvantages of cloning humans. Religion Medical advances Ethics Health of human clones

18. 9.4 Genetic Engineering 1. What types of cloning occur naturally in nature? 2.What are a few of the concerns with cloning? 3.Please write a paragraph explaining the pros and cons of human cloning in your opinion. The Clone Age Questions

19. 9.4 Genetic Engineering The Clone Age Answers 1. What types of cloning occur naturally in nature? 2. What are a few of the concerns with cloning? –bacteria (binary fission- makes a copy of itself) –some simple animals and plants (budding, regeneration) –Identical twins low success rate (approx. 1-3 % are successful) clones “imperfect” and less healthy than original animal decreased biodiversity

20. 9.4 Genetic Engineering Open your biology book to page 225, and write a summary explaining how genetic engineering is responsible for making this mouse glow.

21. 9.4 Genetic Engineering The mouse’s green glow comes from the green fluorescent protein GFP. Scientists put a gene from a glowing jellyfish into a virus that was allowed to infect a mouse egg. The jellyfish gene became part of the mouse’s genes. As a result the mouse cells produce the same protein.

22. 9.4 Genetic Engineering Genetic engineering involves changing an organism’s DNA to give it new traits by inserting cloned genes from one organism into a different organism. –Possible because the genetic code is shared by all organisms (all living things share the same 4 nucleotides A,T,C,G)

23. 9.4 Genetic Engineering Genetic engineering uses Recombinant DNA which is DNA that contains genes from more than one organism. Foreign DNA Original DNA

24. 9.4 Genetic Engineering (bacterial DNA) Bacterial plasmids are often used to make recombinant DNA. –plasmids are closed loops of DNA in bacteria –restriction enzymes cut plasmid and foreign DNA –foreign gene inserted into plasmid –Result: New proteins will be expressed

25. 9.4 Genetic Engineering Plasmids are used in the production of human insulin in diabetics: Human gene that produces insulin is isolated and copied many times Insert the insulin gene into a plasmid to combine it with bacterial DNA Insert plasmid back into bacterial cell Bacteria will produce insulin and multiply quickly Insulin is then gathered an purified Diabetics can inject insulin into their bloodstream to maintain their body sugar

26. 9.4 Genetic Engineering Genetic engineering produces organisms with new traits. A transgenic organism has one or more genes from another organism inserted into its genome.

27. 9.4 Genetic Engineering Jurassic Park Questions Why does Dr. Grant need a drop of blood from Dr. Grant? What is inaccurate about the cloning of Dr. Grant? How do the Jurassic Park scientists manipulate the dinosaur DNA to make transgenic dinosaurs? What type of egg do they use to allow the dinosaurs to develop in? (Very quiet, in the backgound)

28. 9.4 Genetic Engineering Jurassic Park Questions Why does Dr. Grant need a drop of blood from Dr. Grant? To extract DNA from What is inaccurate about the cloning of Dr. Grant? A real clone would not be the same age, it would be a baby How do the Jurassic Park scientists manipulate the dinosaur DNA to make transgenic dinosaurs? They fill in the “gaps” in the dinosaur DNA with frog DNA What type of egg do they use to allow the dinosaurs to develop in? (Very quiet, in the background) They use unfertilized ostrich eggs (This is why there is no need for a surrogate.

29. 9.4 Genetic Engineering The mouse’s green glow comes from the green fluorescent protein GFP. Scientists put a gene from a glowing jellyfish into a virus that was allowed to infect a mouse egg. The jellyfish gene became part of the mouse’s genes. As a result the mouse cells produce the same protein.

30. 9.4 Genetic Engineering Transgenic bacteria can be used to produce human proteins such as insulin –gene inserted into plasmid –plasmid inserted into bacteria –bacteria express the gene and multiply –Protein then produced as medicine

31. 9.4 Genetic Engineering Transgenic plants –transgenic bacteria infect a plant –plant expresses the foreign gene –many crops are now genetically modified(GM) ***In order for plants to “pass down” the genetic trait to their offspring they must be sure that the gene is present in the seed of the plant!***

32. 9.4 Genetic Engineering Farmers use pesticides to get rid of “pests” Farmers use herbicides to get rid of weeds –The problem is that the herbicides that kill the weeds also kill the crops –Scientists have developed genetically engineered plants that are resistant to the herbicides

33. 9.4 Genetic Engineering Transgenic animals are used to study diseases and gene functions. –transgenic mice used to study development and disease –“gene knockout” mice used to study gene function »Some genes are purposely “turned off” The protein leptin which controls food intake has been turned off

34. 9.4 Genetic Engineering In order for animals to “pass down” the genetic trait to their offspring they must be sure that the gene is present in the sex cells of the animal! –Egg –Sperm

35. 9.4 Genetic Engineering Scientists have concerns about some uses of genetic engineering. –possible long-term health effects of eating GM foods –possible effects of GM plants on ecosystems and biodiversity

36. 9.4 Genetic Engineering Bacteria used to produce artificial sweeteners Aspartame- Widely used artificial sweetener –Diet soda –Sugar-free gum and candy –Sugar-free desserts –Sugar-free condiments

37. 9.4 Genetic Engineering Draw a double bubble map comparing and contrasting cloning and genetic engineering. Give examples.

38. 9.4 Genetic Engineering Cloning Genetic Engineering Changes DNA to give it new traits Genetically identically copy of a gene or organism Mouse that has jellyfish DNA Biotechnology May involve manipulating DNA Weather/drought resistant fruits and veggies Dolly the Sheep, CC the cat Uses nuclear transfer