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Warm-up 1/9: Finish Pedigree Worksheet: #11-16

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Presentation on theme: "Warm-up 1/9: Finish Pedigree Worksheet: #11-16"— Presentation transcript:

1 Warm-up 1/9: Finish Pedigree Worksheet: #11-16
Turn in LAB from Yesterday with Karyotype Stapled to written section.

2 Warm-up 1/9: What is a clone? What do you think a DNA Finger Print is?
Biotech Quest Quiz: Tuesday 1/14

3 Biotechnology Notes Chapter 9

4 Biotechnology Scientist change an organism’s DNA to give it new traits. Possible because all organisms have the same Genetic Code

5 What are the benefits? Insert needed gene into an organism
Make better vegetables/fruits Identify a suspect Increase biodiversity

6 Restrictive Enzymes Cuts DNA Act like molecular “scissors”
Lets scientist take out and insert a new gene

7 Restrictive Enzyme Different restrictive enzymes cut in different places They recognize nucleotides between 4 and 8 bases long

8 Restrictive Enzymes Some cut straight and make “blunt ends”
Some cut staggered and make “sticky ends”

9 Gel electrophoresis Uses an electrical current to separate DNA sequences that were cut by restrictive enzymes.

10 Gel electrophoresis Electrical currents pull the pieces through a gel
Smaller fragments can move faster & farther than longer fragments

11 Gel electrophoresis Each piece creates a band on the gel
Creates a DNA fingerprint Everyone has their own unique DNA fingerprint

12 DNA Fingerprinting Made by restrictive enzymes and gel electrophoresis
What is it used for? Paternity tests Evidence in criminal cases Studying biodiversity (mother) (child 1) (child 2) (father)

13 http://player. discoveryeducation. com/index. cfm

14 Plasmid loops of DNA in bacteria
restriction enzymes cut plasmid and foreign DNA foreign gene inserted into plasmid

15 Recombinant DNA Contains genes from more than one organism
(bacterial DNA)

16 Transgenic Organism Has one or more genes inserted in it’s DNA
Has recombinant DNA

17 Transgenic Organism Transgenic bacteria used to make human protein
Gene inserted into plasmid Plasmid inserted into bacteria Bacteria makes the protein the gene coded for

18 Uses of Transgenic Organisms
Transgenic plants used in agriculture Creates crops resistant to frost, diseases, and insects Food produced more quickly and cheaply

19 Uses of Transgenic Organism
Transgenic animals Used to study diseases and gene functions.

20 Concerns about Genetic Engineering
possible long-term health effects of eating GM foods possible effects of GM plants on ecosystems and biodiversity

21 Class work Vocabulary Chart

22 Cloning & Gene Therapy Notes

23 Genetic Screening Test DNA to detect genetic diseases
determines risk of having or passing on a genetic disorder can detect genes related to an increased risk of cancer can detect some genes known to cause genetic disorders DMD N

24 Gene Therapy Is the replacement of faulty genes
Gene therapy replaces defective or missing genes, or adds new genes, to treat a disease.

25 Gene Therapy Has many technical difficulties
Inserting gene into correct cell Determining effect on other genes Only way to cure genetic disease is to change the affected DNA

26 Cloning A clone is a genetically identical copy of a gene or of an organism.

27 Cloning Cloning occurs in nature. bacteria (binary fission)
some simple animals (budding, regeneration) Identical twins

28 Cloning Mammals can be cloned through a process called nuclear transfer. nucleus is removed from an egg cell nucleus of a cell from the animal to be cloned is implanted in the egg

29 Benefits of Cloning Human organ transplants Save endangered species

30 Concerns of Cloning Low success rate Clones less healthy than original
Ethical controversy

31 Jurassic park

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