LC/MS Chromatography Systems 212-LC

2 The Complete Solution Varian LC components, pumps, autosamplers and the New MS Workstation SW (V6.6) can be used to create a complete configuration, allowing full control of the Varian LC/MS system

3 The Complete Solution Software MS Workstation Software Single point control of modules and accessories Effortless detector optimisation and control Simple navigation while reviewing and processing results Network compatibility for file management, printing and remote access Extensive reporting for complete analyses Fully integrated 212-LC command and control driver

4 The Complete Solution Autosamplers Autosamplers Productivity increases with automation of sample injections. A range of modules are offered to suit various throughput needs HTS PAL is a low loss, high speed, high capacity, robust autosampler offering outstanding flexibility and productivity ProStar 430 is a precise, versatile autosampler delivering reliable results, particularly for limited sample volumes ProStar 410 is an economical, versitle and easy to use autosampler that is suited to research use, but rugged enough for routing QC work.

5 The Complete Solution HPLC Columns HPLC Columns Varian offers a variety of high quality columns and media for sample prep to analytical and on to preparative applications. Polaris columns provide unique bonded phases to maximize polar retention and selectivity, while virtually eliminating silonol activity. Polaris sets new standards for peak symmetry, selectivity and reproducibility Pursuit diphenyl columns deliver fast separations without sacrificing resolution. Moving from analytical development to production – Pursuit materials meet requirements for analytical resolution and high quality prep

6 The Complete Solution HPLC Detectors HPLC Detectors Several Varian HPLC detectors are useful for LC/MS applications PDA and UV/Vis offer easy offline methods development and trouble shooting for separations ELSD adds an additional “universal” detection channel to the MS data, finding hidden peaks and giving additional quantitative insights

7 The Complete Solution Chromatograph Pumps 212-LC Chromatography pump To meet the flow requirements of modern LC/MS the use of a binary, low delay volume, high pressure mixing system is essential. The 212-LC pumping system has the ability to meet your needs from fast 4.6 mm columns down to 2 mm columns for bioseparations. Quality gradient or blending separations up to 2 mL/min. With excellent accuracy and stability for compositional, flow and pressure Reliable performance for LC/MS is essential. The mechanical design from the mechanism to no-prime check valves is designed for years of reliable use. Fully integrated software control under MS Workstation assures complete LC/MS control and data storage

8 The Complete Solution LC Performance – Low Delay Volume Low delay volume means effective 200 µL/min gradient runs Parabens (Methy, Ethyl, Propyl, Butyl) five minute gradient run, great precision and fast gradient results with little delay volume

9 The Complete Solution LC performance – Compositional Precision Fine precision of flow and composition make for better peak ID, retention time accuracy and quantitative quality 10% steps, far less than 1% ripple and accuracy values, 200 µL/min

10 List Price Performance Target Position Low Performance Mid Performance High Performance Performance at a Competitive Price

11 The Complete Solution Benefit Bundles Benefit Bundles: Understanding what customers value

12 The Complete Solution Benefits Performance State of Play performance for analytical columns Quality and Uptime Modern, conventional reciprocating design for rugged reliable operation Purchase Price Strong value for performance, small percentage of the LC/MS package price Cost of Ownership ability to run low flows reduces solvent and waste disposal costs Credibility Industry standard specifications and performance allows the focus to move to MS benefits and advantages

13 The Complete Solution 212-LC Ordering Information LC –includes 150 µL gradient mixer, heads, plumbing and cables for MS workstation control Optional Degas strongly recommended for Gradient work –A6313 (4 channel, 1 mL/min) Optional mixers are available on the price list Requires MS workstation 6.6 or higher

14 The Complete Solution Pricing Pricing Two head design with higher performance and lower delay volume than the 210 series –commands an increased value-price Minor part of the LC/MS system pricing 212-LC price is complete –an assembled and working pump for the MS Pricing on SAP or from local sales managers, approximately 20% increase from 210 s

15 Questions Can I convert my existing order ? Shipment: immediately –Only orders with LC/MS will be filled in Q4 –Solo 212-LC orders (for existing MS systems) will ship in Q1 FY07 New orders should be quoted with 212-LC If the modules have not been shipped or built, changes can be processed with the associated pricing changes Other ?

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