1. Varian 940-LC Analytical to Prep HPLC

2. Varian 940-LC Silver Bullets
The 940-LC one instrument – many uses:
High performance automated Analytical HPLC
High performance semi-preparative HPLC
High performance prep LC

3. Varian 940-LC Silver Bullets
Flexible and capable
Wide flow rate from 0.1 ml/min to 200 ml/min
Dual path-length flowcells for extended Abs
One Injector, analytical or prep volumes (5ul to 4.5ml)
Superb fraction collecting
Galaxie real-time peak detection
Practical collect and inject
Much Easier interface, it does what you tell it!
Cleaner UI

4. Goals of the 940-LC Analytical to Prep
Aim
Become market leader in this $180M segment
Double market share
Market
Continue to focus on Pharma / BioPharma
Academic
Strategy
Deliver innovation in hardware, consumables, software and services

5. Varian 940-LC Instrument Overview
Factory tested, pre-plumbed instrument:
Plug and play operation
High performance semi-preparative HPLC
Gradient Analytical HPLC
Autoprep System
Choice of UV or PDA detector
Dual path flow cell for unmatched range and scalability
No compromise performance for prep and analytical – switch with a key stroke – no plumbing or hardware changes needed!
The most flexible analytical to prep HPLC available
Fast prep
Integ fc co;\llection
Perform
Consumables
Ana to skid

6. Varian 940-LC Instrument Overview
Scale-up Module for automated methods transfer from analytical to semi-prep, or switching from Analytical to Prep work
Versatile and robust solvent-delivery system
New loopless autosampler – wide volume range
NEW Fraction collection with
Complete range of collection modes such as slope, threshold and time slicing
Wide range of vessels, including autosampler rack
Comprehensive range of columns and consumables
More productivity via GalaxieTM software
Valve automation for genuine unattended operation
NEW direct control interface

7. Varian 940-LC Versatility and Performance
1 ml/min
21 ml/min
Easy to change pump heads
Versatile solvent delivery systems
Powerful pumps allow 4000psi at 25mL/min operation for fast chromatography
Ideal for 4.6mm and 1” column
Able to use smaller 3 & 5 um particles columns at elevated pressures
Improves productivity
Save solvents and waste costs
Short run times save time
High resolution separations = high purity
We have uncovered the market need -

8. Varian 940-LC Pumps Same range of accessories as PS210/218
5ml Head to 200ml Head
Easily interchangeable pressure modules thru removable side panel.
Easy access to mixer
Self maintenance and versatility features have been completely retained with no compromises – integrated systems are notoriously difficult to maintain so this is Unique

9. Varian 940-LC Pumps - FAB Feature Advantage Benefit
Interchangeable Pump Heads
(5 to 200ml/min)
Unrivalled flexibility for any application
Economical and Convenient with Excellent Performance
Open access for ease of maintenance
Easy for the user to correct basic issues resulting in more uptime
Dependable
Simple and system Durability
Analytical to Prep on one System
One system will do the work of two providing high performance flexibility
Performance and Reliability at an Excellent Price/Performance ratio

10. Varian 940-LC Autosampler
Prep version of the 920-LC Sampler
5ul to 4.5ml Injection range
No necessity to combine a Manual injector
No need to dedicate injector to prep or analytical
One Sampler does all!
Cooled version
Loopless Design
Needle in path Design
No carryover
2008 Technology – no changing loops

11. Varian 940-LC Autosampler - FAB
Feature
Advantage
Benefit
Needle in Path
Design
No need for Injection loops
No Carryover
Versatility, Economical
Performance
One Injector, large or small inject volumes
Simplifies the system, no need to swap components for analytical to prep
Accuracy and Seamless Simplicity
One Autosampler, all rack formats
No need for multiple injector options
Versatility
Simplicity

12. Varian 940-LC UV/Vis - PDA Detectors
High Sensitivity for Analytical applications
Extended absorbance – desensitised, for Prep Applications
All in One Cell!
NEW 4 x 0.25mm Cell low dead volume
Excellent performance at low flow rates for Analytical
Excellent Extended Absorbance for Prep

13. Varian 940-LC Dual Pathlength Flowcell
Absorbance Detector
Extended absorbance range without re-plumbing (<70 AU)
NEW 4mm x 0.25 mm
4mm x 0.15 SuperPrep
Low dispersion
UV/Vis Detector
PDA Detector
Low noise ~ +10 mAU
Low Drift ~ 1 mAU/hr

14. Agilent 1200 Flowcells

15. Varian 940-LC UV/Vis - PDA Detectors - FAB
Feature
Advantage
Benefit
NEW 4x0.25 Low Volume Cell
Optimised for Analytical to Prep, no need to change Cells
Performance and Versatility and Dependable
Tool Free maintenance
Easy to change lamps and flow cells
Simplicity
Range of cells for ul/min to Litres/min
Fits all applications from Analytical to Benchtop Prep
Versatility and Performance

16. Varian 440-LC Fraction Collector
Fully controlled by software for foolproof collection by slope, level or timeslice
Known fluid path for accurate fraction collection
Software display of collected fractions and source injections
Reliable operation
Compatible with a wide range of containers
Belart and other optional racks
Microvial to 50ml tube capacity
Autosampler rack compatibility provides practical collect and re-inject capabilities

17. Varian 440-LC Fraction Collector
We chose not to collect Peak 3
Peaks 1,2,4,5,6,7 collect with exceptional accuracy
Excellent algorithm, and simple delay volume calculation
Slope/Threshold based on real numbers!!

18. Varian 440-LC Fraction Collector
Fraction Log shows peak location using colour coded rack assignment

19. Varian 440-LC Fraction Collector - FAB
Feature
Advantage
Benefit
Modern Design, improved movement and hardware
Significant improvement ergonomically on 701
Pleasing Appearance with Stylish fast Collector movement
Simplified user interface
Easy to use, does what you ask it to do both manually and automated
Simplicity and precious Dependability for customer fractions
Inject/Collect and Re-inject
Collect into A/sampler rack then re-insert into the Sampler for Fraction Purity Determination
Provides Confidence in Fraction Puritys without the need to do any liquid transfers

20. Varian 445-LC Scale-up Module
Optimized flow paths for automated scale-up
Separate optimised flow paths for Prep and Analytical columns
Solvent selection valves for 4 solvents
( 2 per pump)
Integrated priming valves for easy solvent changes
Two Integrated Push Button Manual valves for Analytical and prep Injections

21. Varian 445-LC Scale Up Module( SUM) - FAB
Feature
Advantage
Benefit
2 Solvent Selection valves for solvent switching and flexibility
Can prime up 4 most popular solvents thus saving time on re-priming
Simplicity and Dependability
Analytical and Prep Columns Positions for Automated Scale Up or Switching
Seamless and simple transfer from an Analytical analysis to Prep
Simplicity and Versatility
Integrated Manual Valves which are dedicated for Analytical and Prep
Push button valve positioning with automatic return, eliminate operator error
Even Simpler to use, eliminates user error improving Confidence in results

22. Varian 940-LC Software Usability
Software designed for Prep users
Enhanced manual control
One page status and chromatogram
Real time fraction collection display
Advanced peak sensing for better fraction collection
Purity displays for absorbance detection – standard!
Documentation and audit trails for traceability and regulatory needs

23. Varian 940-LC Configurations
pump
inject
detect
SUM FC
Modular
Isocratic
25ml
Opt any
Manual
UV/Vis NA
Base
Grad
Man inj
Dual UV
option
Productive 1
Opt 50
Auto 5ml +man
yes
Productive 2
PDA
Prep
100ml
Opt 200
manual
Dual UV/Vis

24. Varian 940-LC Consumables
Columns designed for Prep users
From 3 to 10mm media
Wide range of C4 to C18
Silica and Polymer media
From 2mm to 2” ID
Unique solutions for dedicated prep with load and lock systems
Matched Analytical to Prep columns and packing's to assure smooth scale up
Solutions for hardware, software and consumables

25. Varian 940-LC Summary The 940-LC one instrument – many uses
High performance automated Analytical HPLC
High performance semi-preparative HPLC
Versatility
Pumping system able to operate at analytical flow rates and scalable up to prep flows
Dual path-length flow cell technology for unmatched range
Scale-up Module for automated methods transfer
Advanced fraction collection with full software control
Complete range of columns and consumables
Prep enhanced Galaxie TM software and valve automation for genuine unattended operation

26. Conclusion for 900 Series Compact and Stylish Integrated Systems
Retain flexibility and maintenance features
Top Tier hardware performance
Top Tier User Interface – pleasant experience
Simplicity in a box – Install , Use and Maintain
1st Truly Integrated HPLC’s on the Market 26 26

27. Questions??