1. HPLC Equipment : Detectors
Analytical Chemistry Lab, Department of Chemistry, Yonsei University

2. Characteristics of Ideal Detector
Have high sensitivity and the same predictable response
Response to all solutes or else have predictable specificity
Have a wide range of linearity
Be unaffected by changes in temperature and mobile phase flow rate
Response independently of the M.P.

3. Ideal Detector Cont’d Not contributed to extra column band broadening
Be reliable and easy to use
Have linear response to solute
Be non-destructive
Provide qualitative and quantitative information
Have a fast response

7. Characteristics of UV-VIS
Capable of Very high Sensitivity (must UV-VIS absorption)
Good linear range (~ 5 order)
Very small cell volume (low band broadening)
Relative insensitive to M.P. and temperature change (except very high sens)
Very reliable and easy to use

8. UV-VIS cont’d 가장 흔하게 사용되는 검출기죠
Wide varying response for different solute
Gradient elution capability
Wave length can be selected
Non-destructive to sample
가장 흔하게
사용되는
검출기죠

15. Characteristics of RI detector
Excellent versatility – universal
Moderate sensitivity (not for trace analysis)
Efficient heat exchanger required
Relatively insensitive to flow change
Sensitive to type of M.P. and temp change
Reliable, fairly easy to use
Non-destructive
Difficult to use with gradient elution

17. Flourescence detector
High sensitivity
Small sample size
Selectivity but
not versatility

23. LC-MS For most compounds, a mass spectrometer is
more sensitive and far more specific than all
other LC detectors. It can analyze compounds
that lack a suitable chromophore. It can also
identify components in unresolved chromato-graphic peaks, reducing the need for perfect
chromatography.

25. Ion Source Electrospray ionization (ESI)
Atmospheric pressure chemical ionization
(APCI)
Atmospheric pressure photoionization (APPI)

26. Difficulties on LC-MS
The gas loaded to the vacuum system resulting from evaporating the M.P. is generally too high
The M.P. composition often imcamparable with M.S.
The transfer of polar and ionic analytes from the liquid to the gas phase is difficult

28. MC-MS operational 주의 사항