1. Solution Preparation Solvent Mixing

2. The foundation of analytical chemistry is accurate, documented solution preparation and solvent mixing.

3. PhasePrep is an instrument designed to document and prepare solutions and mix solvents for HPLC mobile phases, titrations, extractions etc. GMP Compliant.

4. Why prepare solutions gravimetrically rather than volumetrically?

5. The gravimetric method is inherently more accurate than the volumetric method because the temperature of the solvent can be ignored. The amount of solvent contained by a volumetric flask is a function of temperature—but the weight of the solvent is not affected by temperature.

6. Safety is improved when using the gravimetric method because the pouring and spillage associated with the volumetric method is reduced dramatically or eliminated.

7. What are the costs of solvent mixing? The cost of loosing laboratory integrity. This is the highest cost of all. What price can be put on mis-identifying a peak in a chromatogram because the solvent was not mixed accurately or a mislabeled solvent was used? The PhasePrep II prepares mixtures to an accuracy of a few hundreths of one percent or better—every time. A label is also printed to document the preparation. This minimizes the cost of re-works, non-conformance investigations and the costly delays that could result in not shipping finished product. The cost of purchasing, replacing and cleaning of volumetric glassware. With the PhasePrep II this is replaced by the cost of uncalibrated solvent containers. The cost of wasted solvent. With the PhasePrep II, 135 ml or 653 ml of a solution can be prepared as easily as one liter. Solvent waste is minimized. The safety cost of handling solvents. The PhasePrep II is a closed system. The exposure to spilled solvents, broken glassware and harmful solvent vapors is reduced.

8. Features and Benefits The proven, self-priming solvent pumps can handle virtually any solvent and can dispense a few drops or deliver solvent at a rate up to 500 ml/minute. As little as 10 ml or as much as six or seven liters of solution can be prepared in a few minutes. The integrated, industrial grade, touch screen computer has no hard disc drives, no external keyboard, no mouse. Up to 100 solution preparation or solvent mixing methods can be easily entered and recalled on the touch screen. For added security all methods and important system parameters are password protected. Up to 20 different operators can access the system through assigned passwords. A total of eight different pumps can be added to the PhasePrep II. Any of three solvents and a solute (or buffer) can be added to a solution or solvent mixture. The integrated, 8.2 kilogram capacity weighing module (Sartorius Mechatronics, Germany) measures the weight of the dispensed solvent to a resolution of 0.01 gram (~10 microliters). Each preparation is automatically labeled. Methods can be easily written for preparing solutions on a g/ml or a g/g basis. Solvent mixtures can be configured for either weight percent or volume percent of a two or three component mixture.

9. All methods and important system parameters are password protected. Up to 20 different operators can access the system through assigned passwords. The integrated, industrial grade, touch screen computer has no hard disc drives, no external keyboard, no mouse. Each preparation is automatically labeled. The user can specify the fields to include. Security, Safety & Recording Features

10. 1.Operator enters the sample number and his/her initials. 2.A sample bottle is placed on the balance and the compute records the weight. 3.Operator is prompted to add the appropriate amount of solute to the bottle. 4.The computer calculates the exact amount of solvent required. The solvent, with the internal standard dissolved, is added to the sample to obtain a dilution with a typical accuracy of 0.02%. 5.The completed sample is removed and the printed sample label is affixed to the bottle. The Process

11. 1.Operator enters the sample number and his/her initials. 2.A sample bottle is placed on the balance and the compute records the weight. 3.Operator is prompted to add the appropriate amount of solute to the bottle. 4.The computer calculates the exact amount of solvent required. The solvent, with the internal standard dissolved, is added to the sample to obtain a dilution with a typical accuracy of 0.02%. 5.The completed sample is removed and the printed sample label is affixed to the bottle. The Process

12. 1.Operator enters the sample number and his/her initials. 2.A sample bottle is placed on the balance and the compute records the weight. 3.Operator is prompted to add the appropriate amount of solute to the bottle. 4.The computer calculates the exact amount of solvent required. The solvent, with the internal standard dissolved, is added to the sample to obtain a dilution with a typical accuracy of 0.02%. 5.The completed sample is removed and the printed sample label is affixed to the bottle. The Process Methods can be easily written for preparing solutions on a g/ml or a g/g basis. Solvent mixtures can be configured for either weight percent or volume percent of a two or three component mixture.

13. 1.Operator enters the sample number and his/her initials. 2.A sample bottle is placed on the balance and the compute records the weight. 3.Operator is prompted to add the appropriate amount of solute to the bottle. 4.The computer calculates the exact amount of solvent required. The solvent, with the internal standard dissolved, is added to the sample to obtain a dilution with a typical accuracy of 0.02%. 5.The completed sample is removed and the printed sample label is affixed to the bottle. The Process

14. 1.Operator enters the sample number and his/her initials. 2.A sample bottle is placed on the balance and the compute records the weight. 3.Operator is prompted to add the appropriate amount of solute to the bottle. 4.The computer calculates the exact amount of solvent required. The solvent, with the internal standard dissolved, is added to the sample to obtain a dilution with a typical accuracy of 0.02%. 5.The completed sample is removed and the printed sample label is affixed to the bottle. The Process

15. Specifications Typical solvent dispensing precision: +/- 2 drops (one drop is approximately.025 g) Solvent weighing precision: 0.01 grams (about 10 microliters) Balance Capacity: 8,200 grams Stores 100 methods Number of users: 20 users with unique passwords. Number of pumps: Can be configured with 2 to 8 solvent pumps Pump type: Pulseless, self-priming, magnetically-coupled gear pumps. No rotating seals. Wetted materials of construction: Teflon©, 316 stainless steel, graphite Maximum Solvent delivery rate: 500 ml/min each pump Touch Screen Computer: PC with Windows XP operating System Electrical: 110-240 Volts @ 50-60 Hz, 100 Watts. Dimensions: 27 cm (10.7”) wide X 52.8 cm (20.8”) long X 34.7 cm (6.9”) high.

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