1. VARIAN, INC. HPLC Products
Wide range of products in analytical LC
High performance analytical instrumentation
Choice of workstations
Extensive range of columns and consumables
Strongest sales and support team
Excellent products and position in Preparative LC
Custom prep skid systems
Flexible instrumentation
Solutions for Biochemists and Biologists

2. Widest Range of Pumps Products in HPLC
Analytical Pumps
ProStar 210 Low cost isocratic analytical
ProStar 220 isocratic analytical
ProStar 230 ternary gradient analytical
ProStar quaternary gradient analytical
Preparative and Semi Preparative LC pumps
210 - Low cost isocratic semi prep
PrepStar Low cost isocratic semi prep
PrepStar 210/218 binary and ternary gradients to semi prep
PrepStar SD-1 - Prep and scale up
PrepStar SD-2 – Low cost prep pump

3. Widest Range of Detector Products in HPLC
UV-Vis detectors
ProStar high performance dual wavelength analytical/prep
ProStar 335 PDA detector
Other Detectors
ProStar 363 Fluorescence
ProStar 355 RI

4. Widest Range of Sample Handling Products in HPLC
Autosamplers
410 - High performance
420 - Flexible, high performance
430 - Well plate AutoSampler
Fraction collector
701 - High performance, flexible

5. Widest Range of Prep Products in HPLC
500 CVM - Automated valving system
510 Column oven
530 Fluidics module
RamPak column packing stations
Lock and Load columns

6. Configured systems
Polaris – Low cost educational system

7. Widest Range of Software Products for HPLC
Galaxie – Client server multi PC, multi instrument network system
Instrument Control, Data Handling, Custom reports
ReSponder software – single PC, 1 instrument
Simple graphical control for prep, data acquisition but no data handling

8. ProStar 220/230/240 Solvent Delivery Systems
Ease of use
No need to degas or He sparge
Simple pump priming
Reliability
Inlet valves do not fail
Older designs have been working 20+ years
Performance
Gradient performance at all flow rates
The ProStar 240 is shown here with the door on. The door hides all of the hydraulics yet is easy to remove. The customer can either keep the door on the unit for a cleaner looking appearance or he can take the door off when he wants to work on the hydraulics or just if he wants to keep the hydraulics visible in case of leaks. The Mast which can currently be mounted on the SD-200/300 pumps, can now be mounted on all of the ProStar 200 series pumps. In this way, any injectors or other valves can be attached to the system at a minimum of cost.
The new ProStar 220 is the old 9002, the new ProStar 230 is the old 9012 and the new ProStar 240 is the old 9012Q. There is still inert versions of the 9012.

9. ProStar 230/240 Solvent Delivery Systems
Mechanical Inlet Valve Design for improved flow delivery & reliability

10. ProStar 210/218 Solvent Delivery Systems
The is a picture of the old SD-200/300 system and the new ProStar 210/215 series. The new pump is smaller in width, longer and slightly Taller. It is the same width as the old 9012 and can stack with either of the new detector as well as the AtuoSamplers. The key pad is slightly smaller but it contains all of the keys that are on the present system. (the ~ key has been replaced with a key with the name SETUP.) An easily removable door fits over the hydraulics on the right side. When the door is one, the system looks very sleek and modern. With the door off, all of the hydraulics are easy to get at.
The pressure is installed on the right side of the pump, In addition, the mixer is installed in the same bay as the pressure module on the right side of the 2nd pump when two pumps or more are used.
The old SD-200 is now the ProStar 210 and the SD-300 is the ProStar 215

11. Different sizes for different flow rates Different materials
210/218 Pump head
Different sizes for different flow rates
Different materials
Optional piston wash
Different pressure ratings (dependent on pump type)

12. Max flow Min flow 5 mL 10 µL 10 mL 10 µL 25 mL 25 µL 50 mL 50 µL
Pump Heads Available
Max flow Min flow
5 mL µL
10 mL 10 µL
25 mL 25 µL
50 mL 50 µL
100 mL 100 µL
200 mL µL

13. Features, Benefits and Values
Feature Benefit
Prep and analytical Lower cost
Built-in ADC Lower cost
Choice of materials Biocompatible
Low price isocratic Affordable

14. PrepStar SD-1 pump

15. PrepStar SD-1 Pump Design
Twin pump heads
Independently driven motor on each pump head
Independent pressure transducer on each pump head controls flow independently
No pulsation throughout the entire flow range
NO PULSE DAMPENERS REQUIRED
Solvent compressibility
Flow accuracy mL/min (0.1 mL/min for 2 largest heads)
Flow precision ml/min (0.01 ml/min for 2 largest heads)

16. PrepStar SD-1 Pump Benefits
Choice of 5 pump heads covering range of flows
50, 200, 500, 800 and 3200 ml/min
Pump heads made of either stainless steel or titanium
Typical arrangement with 200 ml/min heads for best accuracy in 0.01 ml/min up to 200 ml/min range
Ideal for analytical to prep scale up applications
Optimize separation using inexpensive small diameter column at 1 ml/min
Switch valve to divert flow to preparative column packed with identical material at 200 ml/min
Low pump pulsations increases column lifetime

17. PrepStar SD-2

18. PrepStar SD-2 Features and Benefits
Low cost system
Performance for real prep customers
Controlled by ReSponder Prep software

19. ProStar 325 Dual Wavelength UV-Vis Detector
Outstanding performance
Lowest noise of any dual wavelength detector
Dual wavelength with ratio capability
Widest dynamic range from 50 mAU to 70 AU
Handheld computer available to monitor chromatogram in real time, create and edit methods
Compatible with Galaxie and Star WS
Improved system monitoring

20. 325 Optical design

21. ProStar 325 Controlled Optical Imaging
Unique Advantage of COI
Minimize RI effects due to
gradient
injection
solvent
temperature
The flow cell has 5 lenses designed to focus the light directly through the center of the flow cell at all times. An image of the monochrometer exit slit is focuese on the front and rear of the flow cell. Even when a different solvent passes through the flow cell the images only move a short distance from their original position. Because both images are in focus all of the light must pass through them. This insures that none of the light will strike the flow cell walls causing flow or gradient sensitivity.

22. Best noise specification Excellent sensitivity
325 Features and Benefits
COI
No RI response to temperature, solvent or flow
Flatter baselines
Better data handling results
Best noise specification
Excellent sensitivity

23. Flow cell - diagram

24. ProStar 335 Photodiode Array
Outstanding performance
Programmable slit widths 1, 2, 4, 8, 16 microns optimizes noise or resolution
Excellent low noise operation < 1 x 10-5 AU
Monitor all l from 190 to 950 nm
Peak purity and spectral search available
Available in 2 versions
Analytical (single array)
Prep (dual diode)

25. ProStar 335 PDA Optics

26. ProStar 335 Features and Benefits

27. Outstanding performance
ProStar 355 RI Detector
Outstanding performance
Active thermostating gives flatter baselines
Easy to use autozero improves analytical flexibility
Auto purge for automated runs
Wide dynamic range to 512x10-6 RIUFS
Compatible with Galaxie and Star WS

28. ProStar 363 Fluorescence Detector

29. ProStar 363 Optics Diagram

30. ProStar 363 features and benefits
Feature Benefit
Reference diode Low noise
Intense lamp High signal
Scanning Find optimum wavelength
Computer control Easy to use

31. ProStar 410 – Most Common – 73%
ProStar AutoSamplers
ProStar 410 – Most Common – 73%
Most common AutoSampler sold, 2 different vial sizes, Prep injections, standalone reuse of standards
ProStar 430 – Bio – 17%
Well plate AutoSampler

32. ProStar 410 Autosampler

33. ProStar 430 Autosampler
Automation of 96 or 384 well-plates

34. ProStar 510 Column Valve Module
Outstanding flexibility
Valving for EVERY need
Simple, neat handling of analytical and semi-prep columns
Full software control
Solvent compatibility check
Compatible with Galaxie and Star WS

35. PrepStar 530 – Fluidics Module

36. PrepStar 530 capabilities
Interfaces the PrepStar SD-2 to ReSponder
Solvent selection for high pressure gradients
4 A and 4 B solvents
Used with 2 pumps (SD-2, SD-1, PrepStar 218)
Solvent selection for low pressure gradients
Use 1 pump only, only SD-2
Form stepped and linear gradients.
Fraction collecting
11 fractions
Unique valves for high flow rates
“Blow out” feature puts all sample into collection vial

37. PrepStar 530 capabilities
Recycle
Time program detector output back into pump and column
Sample injection
Inject sample through pump
Detector input to ReSponder software

38. ProStar 701 Fraction collectors

39. RamPak Column Packer

40. ReSponder – software for prep in the factory Galaxie
Workstation
Star
Controls all mod and does full data handling
ReSponder – software for prep in the factory
Galaxie

41. Positioning the ProStar and PrepStar
Widest range of products in HPLC
Excellent products and position in Preparative HPLC
Custom prep skid systems
Choice of workstations
High performance analytical instrumentation
Flexible instrumentation
Solutions for Biochemists and Biologists

42. Full line of prep products - 1
210 pump with larger heads
Occasional semi prep
218 pump with larger heads 200 mL/min
Routine semi prep
SD-1 pump
Scale-up to prep
SD-2 pump
Bench top prep
325 detector
Dual path for extended range
Super prep flow cell
335 PDA with prep flow cell
Diode array prep capability

43. Full line of prep products - 2
701 Fraction collector
Flexible, controlled by the Galaxie workstation
500 CVM with prep and diverter valves
Occasional fraction collecting capability – lower flows
530 Fluidics module
Low pressure gradient with SD-2
Solvent selector
Fraction collector
Sample injection
Recycle

44. Full line of prep products -3
410 AutoSampler
Prep injection capability
Injection with 210/218 pump - AutoPrep
Inject any volume
Peak tracking software in Star
Complete package for prep
ReSponder software
Simple prep software
Control of the PrepStar 530
RamPak column packing stations
Load and Lock columns

45. Custom large prep systems
Up to 10 L/min
Up to 16 in. diameter columns
Custom designed and installed PCG ( Prep Chromatography Group) in Wakefield

46. Flow Cell - Dual optical path
9mm and 1 mm
4 mm and 0.15 mm super prep cell
Extends linear range of the detector to greater than 10 AU
Automatic switching between the two paths at high absorbance