1. Diagnostics – Quaternary Pump & Fraction Collector Analytical 920 LCSemi-Prep 940 LC

2. Modules

3. Quaternary Pump Problems General Issues Reproducibility Carryover No Peak Observed Mixing Ratio Accuracy Pressure Fluctuations Split Peaks Liquid does not Flow No Power Pump Motor does not Rotate

4. Quaternary Pump Problems Degasser Issues Bubbles Generated at Outlet Bubbles Generated when Pump sucks Liquid does not flow Vacuum Pump does not work Vacuum Pump does not stop

5. Prep Pump Problems General Issues Reproducibility Carryover No Peak Observed Mixing Ratio Accuracy Pressure Fluctuations Split Peaks Liquid does not Flow No Power Pump ID’s (broken Spring)

6. Quaternary Pump Electronics Electronics Motor Error Leaks Data & Board Errors Limit Errors

7. Quaternary Pump Electronics

8. Prep Pump Electronics Electronics Fuse on Main Board Pot Adjustments (if applicable) Circuit Cycling Limit Errors

9. IMS Quaternary Pump Actions Inlet check valve replaced Outlet check valve replaced Piston seal replaced Drain valve seal replaced Drain valve filter replaced Solenoid valve replaced Mixer filter replaced Solvent filter replaced Gradient four way joint replaced Cam Position Adjustment

10. IMS Quaternary Pump Tests Flow rate accuracy - Test meter method Flow rate accuracy test - Time volume method Flow Compositional accuracy test Flow Leak test Flow precision test Flow pulsation test Flow compositional Ripple Test Flow gradient linearity test Flow compositional precision test

11. IMS Prep Pump Actions Pump A pistons seal replaced Pump A inlet check valve replaced Pump A outlet check valve replaced Pump B pistons seal replaced Pump B inlet check valve replaced Pump B outlet check valve replaced Solvent inlet filter replaced Mixer filter replaced Zero pump pressure Reset Pump ID

12. IMS Prep Pump Tests Flow Rate Accuracy Test – Flow Meter method Flow Rate Accuracy Test - Time volume method Flow Leak Test Flow Precision Test Flow Gradient Linearity Test Flow Compositional Precision Test Flow Compositional Accuracy Test

13. Pump Practical Person 1: Replacement of the Pump Seal & Drain Valve Seal and Solvent Filter Wash Person 2: Replacement & Cleaning the Inlet and Outlet check valves and Pressure Test Mode in IMS, for 940 people, reset pump ID Person 3: Remove the pump and Pump Board Cam Position Adjustment after Board Replacement