Transcription and Translation

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End Show Slide 1 of 39 Copyright Pearson Prentice Hall Biology Protein Synthesis: I will understand the general pathway of transcription and translation.
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Presentation transcript:

Transcription and Translation Essential Question- How is information moved between nucleic acids and proteins?

Transcription is… The process of how a section of DNA is copied into the complimentary sequence of RNA

1st step of gene expression (turning DNA sequence into functional molecules) Occurs in the nucleus of eukaryotes

Messenger RNA (mRNA) - Short pieces of RNA that carry messages from the DNA to the rest of the cell, instructions for making proteins Ribosomal RNA (rRNA) - make up ribosomes, the organelle that synthesizes proteins ­­­­­­­Transfer RNA (tRNA) - transfers the amino acids to the ribosomes

RNA is made by RNA polymerase that links together nucleotides AND opens the DNA helix

Steps of Transcription Initiation- RNA polymerase binds to and opens the DNA Elongation- RNA strand is made Termination- RNA polymerase comes off the DNA and the DNA helix closes These are the same steps as DNA replication

RNA polymerase makes new RNA strand in the 5’→3’ Template strand- DNA strand used as a pattern for RNA synthesis

At the end of transcription, mRNA moves from the nucleus to the cytoplasm Translation occurs at a ribosome and makes a new protein

Translation The process of ribosomes decoding an mRNA molecule to make a specific protein

Codon- 3 bases that code for an amino acid on an mRNA tRNA (transfer RNA) – RNA molecule that brings free amino acids to the ribosome Anticodon- 3 bases on the tRNA which pairs with the mRNA temporarily

Steps of Translation 1. mRNA moves through the ribosome 2. tRNA with the complimentary anticodon binds to the mRNA codon 3. Ribosome links together the amino acids and releases the empty tRNA 4. Ribosome moves over 3 bases (Steps 2-4 repeat until entire mRNA sequence has been read) 5. When the ribosome reaches a stop codon, the new protein is released and the ribosome releases the mRNA

How many codons are there? 4 4 x x 4 = 64 possible different codons 61 codons for amino acids and 3 stop codons Most amino acids have several codons Remember- There are 20 different amino acids!

Using a codon chart Example: AGU makes serine 2. Find the column for the second base of the codon 1. Find the row for the first base of the codon 3. Find the row for the third base of the codon Example: AGU makes serine

DNA Sequence- TAC GGG CTA ACG ATC Write the complimentary RNA sequence. RNA Sequence- AUG CCC GAU UGC UAG Determine the protein sequence. Protein Sequence- ___________ ___________ ____________ _____________ Methionine Proline Aspartic Acid Cysteine