Presentation on theme: "Restriction Enzymes Enzymes that CUT"— Presentation transcript:
1 Restriction Enzymes Enzymes that CUT DNA at a specific nucleotide sequenceMay produceblunt ends orsticky ends.Sequence cut is a palindrome - same letters forward and backwards!
2 Blunt End Cuts Used to isolate a fragment of DNA Used to cut DNA into pieces for DNA fingerprinting
3 Sticky End CutsUsed when one DNA fragment is combined with another DNA fragment to make RECOMBINANT DNAAllow complementary base pairs to join together.
4 Where do Restriction Enzymes come from? Found in bacteriaEnzymes are used to cut and degrade viruses that enter their cellsNamed for species in which R.E. was foundExample: EcoR1 was first R.E. to be isolated from E. coliPicture of the protein moleculeSequence of bases cut by EcoR1
5 Practical Applications Gene for human insulin or human growth hormone placed into a bacterial plasmid.Gene for disease resistance placed into DNA in cells of a corn plant
7 What’s next?We will use this information to model the genetic engineering of the hormone, insulinYou will choose the best restriction enzyme to cut out the insulin gene and place it into the bacterial plasmid