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Published byDaniela Osborne
Modified over 5 years ago
We can use a process called gel electrophoresis to separate the pieces
Figure 20.x1a Laboratory worker reviewing DNA band pattern
Gel Electrophoresis Moves DNA because it is negative Separates DNA fragments based on size The smaller the fragment the farther it will move Can compare DNA from individuals
Figure 20.17 DNA fingerprints from a murder case
Figure 20.9 Using restriction fragment patterns to distinguish DNA from different alleles
Recombinant DNA When a piece of foreign DNA (in purple) is added to a bacterial plasmid (a circular piece of DNA in bacteria), the end result is known as recombinant DNA
How to make recombinant DNA: take a gene (piece of DNA) from a human cell and combine it with a plasmid from a bacteria using restriction enzymes.
Recombinant DNA: making plants glow
GENETIC ENGINEERING. MANIPULATING GENES… Can we make our food taste better? Can we make humans live longer? Can we make X-men like mutants?!? Let’s start.
Genetic Engineering Genetic Engineers can alter the DNA code of living organisms. Selective Breeding Recombinant DNA Gel Electrophoresis Transgenic Organisms.
What we can do with genes
Warm Up THINK – PAIR – SHARE What genetic technologies do you know of that are in existence? How do you feel about their use?
Genetic Engineering changing DNA within an organism.
Unit 8 test Biotech study guide.
DNA Technology Ch. 20 Figure 20.1 An overview of how bacterial plasmids are used to clone genes.
Objective: You will be able to list the positives and negatives of genetic engineering Do Now: Read “Increasing variation” which starts on p. 320 and ends.
The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints
DNA Fingerprinting. We share 99.9% of our DNA with each other. That means the 0.1% of our DNA makes us unique. But that is still is over 3,000,000 differences!
Biotechnology Technology involving the DNA, genes, and, proteins of different organisms. (Chapter 9) DNA Fingerprinting w/ Gel Electrophoresis Selective.
Genetic Engineering Genetic Engineers can alter the DNA code of living organisms. Selective Breeding Recombinant DNA PCR Gel Electrophoresis Transgenic.
Restriction Enzymes Enzymes that CUT
What are Plasmids? Plasmids are circular pieces of bacterial DNA that often contain genes not related to basic life functions Often contain antibiotic.
Technological Solutions. In 1977 Sanger et al. were able to work out the complete nucleotide sequence in a virus – (Phage 0X174) This breakthrough allowed.
Genetic Engineering. What is genetic engineering? Application of molecular genetics for practical purposes Used to – identify genes for specific traits.
Molecular Genetics Lab Review. Bacterial Transformation Genetic transformation—host organism takes in and expresses foreign DNA Genetic engineering—manipulation.
GENETIC ENGINEERING. Scientist use their knowledge of the chemical composition and structure of DNA to study and change DNA This process is known.
Comparing DNA Aim: How can we compare organisms using gel electrophoresis? Do Now: Write two observations from your results of gel electrophoresis.
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