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Production of Human Growth Hormone in Genetically Modified Bacteria.

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Presentation on theme: "Production of Human Growth Hormone in Genetically Modified Bacteria."— Presentation transcript:

1 Production of Human Growth Hormone in Genetically Modified Bacteria

2 mRNA coding for hGR is extracted from human pituitary gland cells DNA copies of the mRNA are made using the enzyme reverse transcriptase cDNA

3 This produces sticky ends Sticky ends (single-stranded ends which will pair up with complementary sequences) cDNA is cut with a restriction endonuclease. (restriction enzyme)

4 Plasmid: small loop of DNA found in bacterial cells Plasmids contain sequences of bases which signal to the cell that this DNA needs to be copied when the cell divides - the origin of replication Origin of Replication

5 Cut plasmid with restriction enzyme to produce fragments with sticky ends Mix with cDNA copies of hGR genes… …complementary sticky ends will pair up by hydrogen bonding

6 Add enzyme, DNA ligase DNA ligase joins two fragments of DNA by making a covalent bond between ends of their phosphate-pentose backbone Recombinant DNA is formed

7 Mix recombinant plasmids with suitable bacteria e.g. E. coli Induce bacteria to take up plasmids Heat shock Electrical shock Chemical treatment

8 Recombinant plasmid is copied as cells divide Bacteria are cultured hGR produced by genetically modified bacteria Modified bacteria produce hGR


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