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Fungal Growth and Sporulation. G. T. Cole. 1986. Microbiol. Rev. 50: 95-132.

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Presentation on theme: "Fungal Growth and Sporulation. G. T. Cole. 1986. Microbiol. Rev. 50: 95-132."— Presentation transcript:

1 Fungal Growth and Sporulation

2 G. T. Cole Microbiol. Rev. 50:

3 From: Fischer-Parton et al J. Microscopy 198 (3): Exocytosis—enzymes (pectinases, cellulases, proteases) to degrade compounds in the environment Endocytosis—removal of excess plasma membrane, recycling membrane proteins, transport of membrane proteins & lipids to vacuole for degradation, uptake of molecules, etc.

4 ? Mannoproteins Glucan synthase Wall-lytic enzymes, protease activators Chitosomes (chitin synthase) Multivesicular bodies Wall substrates Enzyme activators (GTP), inhibitor Delivered from cytosol? Components probably delivered by membrane- bound vesicles Delivered from cytosol? plasmalemma wall Components of wall synthesis at hyphal tip. Modified from Deacon (2006). Fungal Biology.

5 Asexual Sporulation Sporangiospores formed by cleavage of protoplasm in a multinucleate sporangium (Chytridiomycota, Zygomycota, Oomycota) Sporangiospores formed by cleavage of protoplasm in a multinucleate sporangium (Chytridiomycota, Zygomycota, Oomycota) Conidia develop directly from hyphae or from modified hyphal cells called conidiogenous cells (Ascomycota including Deuteromycetes, some Basidiomycota) Conidia develop directly from hyphae or from modified hyphal cells called conidiogenous cells (Ascomycota including Deuteromycetes, some Basidiomycota)

6 Control of asexual sporulation May be triggered by changes in carbon:nitrogen ratio in growth medium May be triggered by changes in carbon:nitrogen ratio in growth medium May require light trigger, e.g. near-ultraviolet light May require light trigger, e.g. near-ultraviolet light Under control of sporulation genes: Under control of sporulation genes: One group involved in switch from somatic growth to sporulation One group involved in switch from somatic growth to sporulation A second group regulates developmental stages of sporulation A second group regulates developmental stages of sporulation A third group regulates secondary aspects including spore color A third group regulates secondary aspects including spore color

7 Asexual Fungi: Deuteromycetes

8 Deuteromycetes “deuter-” Gk., meaning “second” > 20,000 species of fungi in 2600 genera have no known sexual state > 20,000 species of fungi in 2600 genera have no known sexual state Most belong in phylum Ascomycota Most belong in phylum Ascomycota These fungi are also called: These fungi are also called: Anamorphic fungi Anamorphic fungi Mitosporic fungi Mitosporic fungi Conidial fungi Conidial fungi Imperfect fungi Imperfect fungi Fungi imperfecti Fungi imperfecti

9 Asexual propagules Chlamydospore Chlamydospore 1-celled spore (usually thick-walled) designed for perennation; formed inside existing cell 1-celled spore (usually thick-walled) designed for perennation; formed inside existing cell Sclerotium (pl. sclerotia) Sclerotium (pl. sclerotia) Rounded mass of hyphae, often differentiated into rind and medulla Rounded mass of hyphae, often differentiated into rind and medulla

10 Asexual propagules Conidium (pl. conidia) Conidium (pl. conidia) Non-motile spore designed for dispersal Non-motile spore designed for dispersal Wide range of shape, size, color and septation among taxa Wide range of shape, size, color and septation among taxa

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12 Saccardoan Spore Types P.A. Saccardo ( ) P.A. Saccardo ( ) “Sylloge Fungorum” ( )--names & descriptions of all known fungi “Sylloge Fungorum” ( )--names & descriptions of all known fungi Developed system of classifying fungi based on type of spore (shape, septation, color) Developed system of classifying fungi based on type of spore (shape, septation, color)

13 Saccardoan Spore Types Color Color Hyaline or bright (hyalo-) Hyaline or bright (hyalo-) Pigmented (phaeo-) Pigmented (phaeo-) Shape and septation Shape and septation 1-celled—amerospore 1-celled—amerospore 2-celled—didymospore 2-celled—didymospore Multicelled—phragmospore Multicelled—phragmospore Muriform—dictyospore Muriform—dictyospore Filiform—scolecospore Filiform—scolecospore Helical—helicospore Helical—helicospore Branched--staurospore Branched--staurospore

14 Saccardoan Spore Types amerospore staurospore helicospore scolecospore dictyospore didymospore phragmospore

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16 Synanamorph Two or more types of asexual spores formed by the same fungus Two or more types of asexual spores formed by the same fungus Example: Example: Ceratocystis fibriata

17 Conidiogenous Cells A cell that forms one or more conidia A cell that forms one or more conidia May be formed on a specialized simple or branched hypha called a conidiophore May be formed on a specialized simple or branched hypha called a conidiophore

18 From D. Malloch

19 Conidial Development (Ontogeny) Blastic—blowing out of conidial initial prior to formation of delimiting septum Blastic—blowing out of conidial initial prior to formation of delimiting septum Thallic—conversion of segment of existing hyphae into conidia Thallic—conversion of segment of existing hyphae into conidia

20 Blastic versus thallic Cole, 1986

21 Blastic development Holoblastic Holoblastic single conidium is formed from conidiogenous locus, all wall layers involved in formation of conidium wall single conidium is formed from conidiogenous locus, all wall layers involved in formation of conidium wall Enteroblastic Enteroblastic more than one conidium formed from locus, only the inner wall layer(s) involved in formation of conidium wall more than one conidium formed from locus, only the inner wall layer(s) involved in formation of conidium wall

22 From Hawksworth et al., 1995 Holoblastic

23 From Hawksworth et al., 1995 Holoblastic/synchronous Holoblastic/sympodial

24 Enteroblastic development Phialidic—a basipetal succession of conidia is formed from a fixed locus on the conidiogenous cell (phialide) Phialidic—a basipetal succession of conidia is formed from a fixed locus on the conidiogenous cell (phialide) collarette

25 Enteroblastic development Annellidic—a basipetal succession of conidia formed by repeated percurrent proliferation of conidiogenous locus, leaving the distal end of locus with transverse scars (annellations) Annellidic—a basipetal succession of conidia formed by repeated percurrent proliferation of conidiogenous locus, leaving the distal end of locus with transverse scars (annellations)

26 Enteroblastic development Tretic—the inner wall of the conidiogenous cell blows out through a hole (pore) in the outer wall like a balloon to form a conidium. Tretic—the inner wall of the conidiogenous cell blows out through a hole (pore) in the outer wall like a balloon to form a conidium.

27 Arrangement of conidia at locus Solitary Solitary Catenate = true chains Catenate = true chains Seriate = false chains, spore heads Seriate = false chains, spore heads Dry spores Dry spores Wet spores (gleoid) Wet spores (gleoid)

28 Succession of conidia Basipetal = a chain of conidia in which new spores are formed at the base, the oldest conidia are at the apex Basipetal = a chain of conidia in which new spores are formed at the base, the oldest conidia are at the apex Acropetal = a chain of conidia with the new spores formed at the end of the chain, oldest spores are at the base. In order for this type of conidial formation to occur, the conidia must function as conidiogenous cells (e.g., Alternaria, Cladosporium) Acropetal = a chain of conidia with the new spores formed at the end of the chain, oldest spores are at the base. In order for this type of conidial formation to occur, the conidia must function as conidiogenous cells (e.g., Alternaria, Cladosporium)

29 Alternaria

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31 Cladosporium

32 Conidiophores Hyphae bearing conidiogenous cells Hyphae bearing conidiogenous cells Morphologically differentiated from vegetative hyphae (=macronematous) Morphologically differentiated from vegetative hyphae (=macronematous) Morphologically not differentiated (=micronematous) Morphologically not differentiated (=micronematous)

33 Conidiophores

34 Types of Deuteromycetes Hyphomycetes—fungi that produce conidia from conidiogenous cells free on their mycelia Hyphomycetes—fungi that produce conidia from conidiogenous cells free on their mycelia May be formed on the surface of synnemata or sporodochia May be formed on the surface of synnemata or sporodochia Coelomycetes—fungi that produce conidia from conidiogenous cells formed in conidiomata Coelomycetes—fungi that produce conidia from conidiogenous cells formed in conidiomata

35 Synnemata Conidiophores united at base Conidiophores united at base Conidiogenous cells at apex Conidiogenous cells at apex

36 Sporodochium A compact, cushion- like aggregation of hyphae on which conidiophores are formed in a dense layer A compact, cushion- like aggregation of hyphae on which conidiophores are formed in a dense layer The aggregation of hyphae is called a stroma (pl. stromata) The aggregation of hyphae is called a stroma (pl. stromata)

37 Hyphomycetes Moniliaceae—conidiophores formed singly, hyphae and conidia pale-colored Moniliaceae—conidiophores formed singly, hyphae and conidia pale-colored Dematiaceae—conidiophores formed singly, hyphae and/or conidia dark-colored Dematiaceae—conidiophores formed singly, hyphae and/or conidia dark-colored Tuberculariaceae—conidiophores aggregated on cushion-like sporodochium (pl. sporodochia) Tuberculariaceae—conidiophores aggregated on cushion-like sporodochium (pl. sporodochia) Stilbaceae—conidiophores aggregated in a synnema (pl. synnemata), an erect bundle with conidia formed at apex Stilbaceae—conidiophores aggregated in a synnema (pl. synnemata), an erect bundle with conidia formed at apex


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