1. Možnosti evropského výzkumu v oblasti související s fytosanitární problematikou Jana Hajšlová Ústav chemie a analýzy potravin, VŠCHT Praha Jana.hajslova@vscht.cz Národní fytosanitární workshop 3.3.2009, Praha

2. Sustainable production and management of biological resources from land, forest and aquatic environment Activity 2.1: Sustainable production and management of biological resources from land, forest and aquatic environment

3. KBBE-2010-1-2-06: Deepened and enlarged cooperation between phytosanitary (statutory plant health) research programmes - ERA-NET Funding scheme: Coordination and Support Action (supporting action). The requested European Community contribution shall not exceed EUR 1 000 000. requiring enhanced research efforts. The aim is to prevent the introduction into the community of organisms harmful to plants or plant products or their spread within the Community. Due to climate changes and globalization, new threats are continuously emerging and the number and complexity of plant health problems increases every year, requiring enhanced research efforts.

4. KBBE-2010-1-2-05: Integrated pest management in farming systems of major importance for Europe Funding scheme: Collaborative Project (large scale integrating project). The requested European Community contribution shall not exceed EUR 9 000 000. better targeted use of all available pest control measures, including preventive measures, observation and pest monitoring, pesticides and biocontrol agents, modelling and experimentation, including physiological and molecular understanding of plant-pathogen interactions and development of symptoms targeting farming systems in which reduction of pesticide use and better control of pests and pathogens is expected to have major effects at the European scale

5. Jaká jsou rizika pro konzumenty? Je lepší volit bioprodukty?

6.  Samples from Czech market 23 extra virgin olive oils from organic farming (O1 – O23) 23 extra virgin olive oils from conventional farming (C1 – C23)  Method of analysis GCx GC/TOF MS GCx GC/TOF MS EXTRA VIRGIN OLIVE OIL The oil obtained from the fruit of the olive tree solely by mechanical or other physical means under the conditions. particularly thermal conditions, that do not lead to alternations in the oil, and which has not undergone any treatment other than washing, decantation, centrifugation and filtration. OLIVE OIL

7. Sum of 16 PAH = Sum of B[a]A, Chr, CP[cd]P, 5-MeChr, B[c]Fln, B[b]F, B[k]F, B[j]F, B[a]P, DB[ah]A, B[ghi]P, I[cd]P, DB[ah]P, DB[al]P, DB[ai]P, DB[ae]P PAHs in olive oil Benzo(a)pyrene)

8. PESTICIDE RESIDUES in olive oils Acephate Acrinathrin Aldrin Azinfos ethyl Azinfos methyl Beta cyfluthrin Bifenthrin Bromophos ethyl Bromophos methyl Brompropylate Bupirimate Captan Carbaryl Carbophenothion Cyhalothrin Cypermethrin Cyprodinyl Deltamethrin Diazinon Dieldrin Difenoconazole Difenylamin Dichlofluanid Dichlorvos Dimethoate Endosulfan alfa Endosulfan beta Endosulfan sulfat Endrin Ethion Etrimfos Fenamiphos Fenarimol Fenchlorphos Fenitrothion Fenoxycarb Fenthion Fenvalerate Fludioxonil Folpet Formothion HCB Heptachlor Heptenophos Hexythiazox HCH alfa HCH beta HCH delta Chlorfenviphos Chloropham Chlorothalonil Chlorpyrifos Chlorpyrifos-methyl Imazalil Iprodione Isofenphos Kresoxim methyl Lindane Malathion Metalaxyl Methacrifos Methamidophos Methidathion Mevinphos Monocrophos DDD DDE DDT Omethoate Oxyfluorfen Oxychlordane Paraoxon ethyl Parathion Parathion methyl Penconazole Permethrin Phenothrin Phenthoate Phosalone Phoshamidone Phosmet Pirimicarb Pirimiphos methyl Procymidone Propham Pyridaben Quinalphos Sulfotep Tebuconazole Tetraconazole Tetradifon Thiobendazol Tolclofos-methyl Tolylfluanid Triadimefon Triazamate Triazophos Trifloxystrobin Trichlorfon Vinclozolin

9. PESTICIDES DETECTED IN OLIVE OILS

10. Pesticides RESIDUE CONVENTIONALORGANIC n mean (µg/kg) Min - Max (µg/kg) % of positive samplesn mean (µg/kg) Min - Max (µg/kg) % of positive samples Endosulfan sulphate sulphate 2222222255.2 26.9 - 210.2 96833.8 24.5 - 43.5 35 Fenthion8127.1 58.0 - 319 350 - -0 Chlorpyrifos340.5 37.8 - 41.5 13231.7 9 n - number of positive samples

11. komodita produkční systém počet detekovaný ch reziduí mrkev organické 0 0 0 konvenční 2 4 1 jablka organické 0 0 0 konvenční 6 3 6 komodita produkční systém počet detekovanýc h reziduí rajčata organické 0 1 0 konvenční 5 11 9 papriky organické 0 1 konvenční 3 5 3 9 Rezidua pesticidů ve vzorcích z ekologického a konvenčního zemědělství na českém trhu

12. 282 pesticides in LC- MS/MS multiresidue method Supelco Discovery Column: Supelco Discovery C18, Mobile phase: MeOH (A), 0.01M amm. acetate (B) Flow: 0.3 ml/min PERFORMANCE OF METHOD EMPLOYING QQQ: PERFORMANCE OF METHOD EMPLOYING QQQ: for all analytes 4 identification points at concentration levels  0.01 mg/kg available  requirements of 2002/657/EC met Kovalczuk T., Lacina O., Jech M., Poustka J., Hajšlová J.: Novel approach to fast determination of multiple pesticide residues using UPLC-MS/MS. Food Addit. Cont. 25(4), 444–457 (2008).

13. Various contaminants may occur in cereal grains Is it possible to examine the presence of these toxins in a single run? PESTICIDES PESTICIDES MYCOTOXINS MYCOTOXINS PLANT TOXINS PLANT TOXINS POPs POPs PAHs PAHs

14. Pesticides: Mycotoxins: Pesticides and all regulated mycotoxins within single run in 8 minutes (UPLC- TOF MS)

15. AMBIENT MASS SPECTROMETRY NEW …opens the doors to many challenging applications in various areas including food / feed control - Reduced / minimal sample prep - No chromatographic separation

16. Kresoxim methyl Dimoxystrobin Azoxystrobin Picoxystrobin Pyraclostrobin Trifloxystrobin

17. Imazalil in citrus fruit detected in 1 minute Imazalil in citrus fruit detected in 1 minute Cl pattern Elementcomposition direct screening of peel Concentration measured in crude extract by DART TOF MS was 34 µg/kg.