1. Gene of interest 203 base pairs surrounds and promotes transcription of many ( 85%) of GMO genes 225 base pairs Stops the transcription of most GMO genes We will be looking for these fragments of DNA to indicate GMO!

2. Biotech and GMO review for test Place these steps of PCR in the correct order. 1. 2. 3.

3. restriction enzymes PCR electrophoresis plasmid Anneal GMO genetically modified organism Denature nucleotide a process used to separate fragments of DNA by size a sugar, phosphate, base monomer of DNA to stick to to pull apart circular DNA found commonly in bacteria and used often in transgenic scenarios an organism that has had its original DNA modified proteins found originally in bacteria that cut DNA in very specific places A process used to amplify a specific region of DNA

4. 4 degrees Celsius 94 degrees Celsius 59 degrees Celsius 72 degrees Celsius a. hold and refrigerate b. extension of DNA molecule c. denaturing of DNA molecule d. annealing of primers

5. A.PCR standard ruler B. Non-GMO food with GMO primer C. GMO food with plant primer D. GMO food with GMO primer E. GMO-positive control DNA with plant primer F. Non-GMO food with plant primer G. GMO positive control DNA with GMO primer 400 base pairs 200 base pairs