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Published byKristina Clark
Modified over 4 years ago
What are the three steps in PCR?
Denaturation Hybridization of Primer DNA replication
What is the enzyme used for PCR?
DNA Polymerase from a hyperthermophile This is why the enzyme is not destroyed at 94 degrees Celsius
What are introns?
Introns are non-coding sequences found in structural genes in eukaryotes They are removed during splicing
What is reverse transcriptase?
It is an enzyme that copies RNA into DNA It is produced by certain viruses
What are restriction enzymes?
They are enzymes that cut DNA at specific base sequences They are produced by bacteria Many of them cut the DNA so that they produce “sticky ends” or single stranded ends
What are vectors used in making recombinant DNA?
Vectors are self replicating pieces of DNA They are used to get DNA into cells Two commonly used vectors are plasmids and bacteriophage
What are three techniques used to get recombinant DNA into cells?
Transformation Electroporation Microinjection (for eukaryotic cells)
What does RFLP stand for?
Restriction fragment length polymorphism It is often used in DNA fingerprinting It requires gel electrophoresis which separates DNA by size
Ch 12. Researchers can insert desired genes into plasmids, creating recombinant DNA and insert those plasmids into bacteria Bacterium Bacterial chromosome.
Wiki Study Lectures. Conceptually Reading and analyzing double stranded DNA in base pairs compared to single stranded DNA read in Nucleotides Determining.
Definitions: 1. Genetic engineering- remaking genes for practical purposes 2. Recombinant DNA- DNA made from two or more different organisms 3. Restriction.
Genetic Engineering. Tools of Molecular Biology DNA Extraction Cutting DNA Restriction Enzymes Recognize certain sequences of DNA and cut the hydrogen.
Chapter 20~DNA Technology & Genomics. Who am I? Recombinant DNA n Def: DNA in which genes from 2 different sources are linked n Genetic engineering:
Chapter 20 DNA Technology and Genomics
The Clone Age Human Genome Project Recombinant DNA Gel Electrophoresis DNA fingerprints
DNA Technology Chapter 12. Applications of Biotechnology Biotechnology: The use of organisms to perform practical tasks for human use. – DNA Technology:
Chapter 20 DNA Technology. DNA Cloning Gene cloning allows scientists to work with small sections of DNA (single genes) in isolation. –Exactly what.
Chapter 19/20 Viruses/Biotechnology. Lytic Cycle: Virulent Virus replicates genetic material Host cell creates new viruses Host cell breaks & spreads.
1 Genetics Faculty of Agriculture Instructor: Dr. Jihad Abdallah Topic 13:Recombinant DNA Technology.
Genetic modification techniques. Tools of biotechnology Collect DNA Restriction enzymes –Blunt end –Sticky end Ligase enzymes Cloning DNA carriers –Bacteria.
Technological Solutions. In 1977 Sanger et al. were able to work out the complete nucleotide sequence in a virus – (Phage 0X174) This breakthrough allowed.
Today: Biotechnology. Over 600 recent transposon insertions were identified by examining DNA from 36 genetically diverse humans. Tbl 1 Which transposable.
Biotechnology Methods Producing Recombinant DNAProducing Recombinant DNA Locating Specific GenesLocating Specific Genes Studying DNA SequencesStudying.
Chapter 16 Gene Technology. Focus of Chapter u An introduction to the methods and developments in: u Recombinant DNA u Genetic Engineering u Biotechnology.
Genetic Engineering. What is genetic engineering? Application of molecular genetics for practical purposes Used to – identify genes for specific traits.
Class Notes 1: DNA Manipulation. I. DNA manipulation A. During recent years, scientists have developed a technique to manipulate DNA, enabling them to.
Electrophoresis. A process that is used to sort fragments of DNA by placing the digested DNA in a special gel and adding electricity.
Cutting and Pasting DNA The cutters are called restriction enzymes, they cut DNA at specific nucleotide sequences.
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