Chapter 13-2 & 13-3: Manipulating DNA & Cell Transformation

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Presentation transcript:

Chapter 13-2 & 13-3: Manipulating DNA & Cell Transformation Essential Questions: How do scientists make changes to DNA? What happens during cell transformation?

Manipulating DNA Genetic Engineering: making changes in DNA code of organism DNA extraction Open cells, separate DNA Cutting DNA Restriction enzymes: cuts DNA at a specific sequence

Separating DNA Gel electrophoresis: Neg. charged DNA moves toward pos. end of gel Shorter frags. move faster (farther) than longer frags Can be used to compare genomes

Cell Transformation Recombinant DNA Contains DNA from different sources Transforming bacteria: plasmids

Bacteria w/ recombinant DNA can infect plant and animal cells This DNA is integrated into one of the chromosomes of the cell Gene replacement therapy Replace faulty genes with healthy genes to cure disease

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