Question of the Day Question: Give the RNA sequence made from the DNA sequence ATGCTAGCT. RNA DNA Nucleus 1’s Turn in: p. 353 A.Q. 1ab, 2b UACGAUCGA.

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Presentation transcript:

Question of the Day Question: Give the RNA sequence made from the DNA sequence ATGCTAGCT. RNA DNA Nucleus 1’s Turn in: p. 353 A.Q. 1ab, 2b UACGAUCGA

DNA  RNA  Protein Central Dogma of Molecular Biology:

Transcription- creation of RNA using DNA RNA DNA RNA polymerase Nucleus Part 1: Transcription Transcription- making RNA from DNA code in nucleus

Part 1: Transcription

1.Where does transcription take place? 2.What enzyme is responsible for carrying out transcription? 3.What does transcription produce? Discuss With Your Partner: nucleus RNA polymerase RNA

Discuss with Your Partner: What does “translation” mean to you? What are some ways that we could we “translate” the following sentence: When school is over, I am going to hang out with my friends.

Part 2: Translation Translation- using RNA instructions to build a protein

Part 2: Translation Translation- using RNA instructions to build a protein 1. mRNA (messenger) carries code to cytoplasm 2. mRNA is read 3 nucleotides at a time (1 codon) Ex: AUG Codon = 3 nucleotides

Part 2: Translation 3. 1 codon codes for 1 amino acid, which is transferred by tRNA 4. Anticodon part of tRNA must complement mRNA codon Ex: AUG codon (mRNA) & UAC anticodon (tRNA) anticodon amino acid

Part 2: Translation 5. Amino acids linked together by a ribosome to make a polypeptide

Part 2: Translation 6. Polypeptide is folded into a protein Polypeptide Protein

Part 2: Translation

Using codon wheel: – For decoding RNA – Work inside to outside Part 2: Translation

What amino acid does the codon AUG code for? Methionine (start)

What amino acid does the codon GGG code for? Glycine

What amino acid does the codon CAC code for? Histidine

What amino acid does the codon UAG code for? Stop (no amino acid)

Using codon wheel: – For decoding RNA – Work inside to outside – AUG = start codon – UAG, UAA, UGA = stop codons Part 2: Translation

Protein Synthesis Review: Met-Gly-Arg AUG-GGG-CGA-UAG TACCCCGTGATC DNA: mRNA: Protein:

1.What codons… a.are in the RNA sequence AUGCCGUUUGGAUAG? b.What amino acids do they code for? 2.What codons… a.are in the RNA sequence AUGAAUGUUUGG? b.What amino acids do they code for? 3.What amino acids will the DNA sequence TATGCTAGACTG eventually code for?

Put ‘Em In Order! a)tRNA molecules carrying amino acids and begin to match up with codons on the mRNA sequence according to the anticodons on the tRNA molecules b)The polypeptide chain is finished and will later be folded into a complete protein c)The mRNA leaves the nucleus and travels to the cytoplasm through pores in the nucleus d)The amino acids are linked (bonded) together by a ribosome, a protein-building organelle e)DNA is unwound by RNA polymerase and mRNA is created from the DNA sequence according to the base pairing rules