Aberrant regulation of HDAC2 and its association with malignant proliferation of human gastric cancer. Aberrant regulation of HDAC2 and its association.

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Aberrant regulation of HDAC2 and its association with malignant proliferation of human gastric cancer. Aberrant regulation of HDAC2 and its association with malignant proliferation of human gastric cancer. A, HDAC2 expression in normal gastric mucosa, gastric adenoma, and gastric adenocarcinoma as recapitulated by gene expression data from NCBI GEO (GSE24375, GSE13195). *, P < 0.005; **, P < 0.01. B, Western blotting of HDAC2 in a subset of gastric cancer tissues. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as loading control. C, gene and protein expression of HDAC2 in various human gastric cancer cell lines as determined by qRT-PCR and Western blotting. D, mRNA expression of different HDAC isoforms in the human gastric cancer cell line MKN-1 cells determined by qRT-PCR. Data are determined by the 2(-Delta Ct) method and are expressed as the ratio of gene of interest relative to the expression of the housekeeping gene GAPDH. E, the effects of HDAC2 knockdown on cell proliferation for MKN-1, SNU-216, SNU-484, and SNU-719 cells as determined by the MTT assay. To ascertain the knockdown efficiency of HDAC2, protein expressions of HDAC2 were determined by immunoblotting (Top). Jeong Kyu Kim et al. Mol Cancer Res 2013;11:62-73 ©2013 by American Association for Cancer Research