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Wu et al., Supplementary Fig 1 Supplementary Figure 1: The depletion of BRG1 in HeLa cells has no effect on cell proliferation. Sh- empty vector was used.

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Presentation on theme: "Wu et al., Supplementary Fig 1 Supplementary Figure 1: The depletion of BRG1 in HeLa cells has no effect on cell proliferation. Sh- empty vector was used."— Presentation transcript:

1 Wu et al., Supplementary Fig 1 Supplementary Figure 1: The depletion of BRG1 in HeLa cells has no effect on cell proliferation. Sh- empty vector was used as a control.

2 GAPDH BRG1 shVector shBRG1 A. GAPDH HA HA-vector HA-BRG1-WT HA-BRG1-Trunc C. Wu et al., Supplementary Fig 2 B. P<0.02 D. P<0.01 Supplementary Figure 2: A negative regulation of telomerase by BRG1 in human breast cancer MDA-MB- 231 cells. (A) A western blot showing that BRG1 was efficiently decreased by shBRG1. (B) A quantitative RT- PCR determined the hTERT mRNA level in BRG1 depleted cells; shVector was used as a control. (C) HA- tagged BRG1 and BRG1-Trunc with the depletion of ATPase domain were overexpressed in MDA-MB-231 cells. (D) Overexpressing wild-type, but not truncated BRG1 regressed hTERT transcription. P values were calculated using the Student’s t-test.

3 GAPDH BRG1 shVector shBRG1 A. B. C. D. GAPDH HA HA-vector HA-BRG1-WT HA-BRG1-Trunc Wu et al., Supplementary Fig 3 P<0.05 P<0.02 Supplementary Figure 3: A negative regulation of telomerase by BRG1 in embryonic kidney 293T cells. (A) A western blot showing that BRG1 was efficiently decreased by shBRG1. (B) A quantitative RT-PCR determined the hTERT mRNA level in BRG1 depleted cells; shVector was used as a control. (C) HA- tagged BRG1 and BRG1-Trunc with the depletion of ATPase domain were overexpressed in 293T cells. (D) Overexpressing wild-type, but not truncated BRG1 regressed hTERT transcription. P values were calculated using the Student’s t-test.

4 GAPDH Beta-catenin HA HA-Vector HA-BRG1-WT HA-BRG1-Trunc Wu et al., Supplementary Fig 4 Beta-catenin GAPDH BRG1 shVector sh-BRG1 A.B. Supplementary Figure 4: The knockdown and overexpression of BRG1 has a limited effect on β-catenin expression. (A) Western blot determined the abundance of β-catenin in BRG1 depleted HeLa (sh-BRG1) cells and control cells (shVector). (B) HA-tagged BRG1 and BRG1-Trunc with the depletion of ATPase domain were overexpressed in HeLa cells and the expression level of β-catenin was determined by western blot.

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