1. Interaction of NHEJ and HR pathways in PCO cultures.
Interaction of NHEJ and HR pathways in PCO cultures. A, RAD51 foci were assessed as mean foci count per cell in samples after 24 hours of 10 μmol/L rucaparib and 2-Gy irradiation treatment. Foci were counted across three fields of view for each sample counting more than 50 cells in each sample. Results are expressed as fold increase above DMSO-treated controls. DNA-PK protein expression was assessed by Western blotting and normalized to GAPDH housekeeper gene. Error bars are SEM. RAD51 foci and DNA-PK expression results were divided by NHEJ and HR function. B, DNA damage was assessed by γH2AX foci increase and HR function by RAD51 foci increase after 24 hours of 10 μmol/L rucaparib and 2-Gy irradiation treatment compared with DMSO-treated controls. Results were divided by NHEJ and HR function.
Aiste McCormick et al. Clin Cancer Res 2017;23:
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