1. Silencing E4BP4 abrogates the promoting effect of SMAD3 knockdown on IFNγ production in NK-92 cells.
Silencing E4BP4 abrogates the promoting effect of SMAD3 knockdown on IFNγ production in NK-92 cells. A, Real-time PCR result of E4BP4 mRNA expression level in NK-92 cells with or without viral-mediated knockdown of SMAD3 (SMAD3-KD) or E4BP4 (E4BP4-KD). B, Western blot analysis of E4BP4 in NK-92 cells. C, Real-time PCR result of IFNγ mRNA level in NK-92 cells. D, ELISA result of IFNγ production from NK-92 cells. Data represent mean ± SD for three independent experiments. *, P < 0.05; ***, P < versus NK-92-EV; ##, P < 0.01; ###, P < versus NK-92-S3KD cells.
Qing-Ming Wang et al. Cancer Immunol Res 2018;6:
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