1. Cell-cycle regulatory proteins were controlled by O-GlcNAc at FOXO3 S284 through MDM2.
Cell-cycle regulatory proteins were controlled by O-GlcNAc at FOXO3 S284 through MDM2. A, The mRNA expression levels of MDM2-p53 pathway–related proteins (MDM2, p53, and p21) in various FOXO3 mutants were determined by qRT-PCR. B, The expression levels of cell-cycle regulatory proteins (FOXO3, p53, p21, MDM2, CDK2, CDK4, cyclin D1, cyclin E, and GAPDH) in various FOXO3 mutants were determined by Western blotting (see details in Supplementary Fig. S4). This figure was drawn from the information present in the top panel of Fig. 5C. C, This figure illustrates how O-GlcNAc at FOXO3 S284 could activate MDM2 by which p21 is suppressed based on the schematic summary of the LC–MS analysis. **, P < 0.005; ***, P < ; N.S., not significant for indicated comparison.
Heon Shin et al. Cancer Res 2018;78:
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