1. Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis.
Overexpression of miR-335, but not miR-29a, reduces in vitro carcinogenesis. A and B, stable transfection of SBC-5 cells to overexpress miR-335 or miR-29a was achieved using a lentiviral system. Screening of single colonies by qRT-PCR was used to select cell lines with the highest expression of miR-335 (SBC-5 miR-335+) or miR-29a (SBC-5 miR-29a+; n = 3/group) for subsequent studies. Stable SBC-5 cell line transfected with control lentivirus was used as control cell line (SBC-5 VectorCtrl). C and D, compared with SBC-5 VectorCtrl cells, cell migration and invasion were significantly reduced in SBC-5 miR-335+ cells, but not in SBC-5 miR-29a+ cells (n = 3/group, 2 replicate experiments). E, SBC-5 miR-335+ formed fewer colonies than SBC-5 VectorCtrl, whereas SBC-5 miR-29a+ was equivalent to SBC-5 VectorCtrl (n = 3/group, two replicate experiments). F, proliferation, assessed by the MTT assay, was lower in SBC-5 miR-335+ than that in SBC-5 VectorCtrl, whereas proliferation of SBC-5 miR-29a+ cells was equivalent or slightly increased compared with SBC-5 VectorCtrl cells (n = 5/group/time point, 3 replicate experiments; compared with control, *, P < 0.05; **, P < 0.01; ***, P < 0.001).
Meng Gong et al. Mol Cancer Res 2014;12:
©2014 by American Association for Cancer Research