Presentation on theme: "Dr. Kayo Devi Yami RONAST"— Presentation transcript:
1 Dr. Kayo Devi Yami RONAST STATUS, POTENTIALS AND CHALLENGES OF BIOTECHNOLOGY DEVELOPMENT IN NEPALDr. Kayo Devi YamiRONAST
2 TRADITIONAL BIOTECHNOLOGIES For many centuries societies and communities have been using these technologies to alter plants & animal products to produce BETTER FOOD & IMPROVED PRODUCTION without knowing the actual scientific background.DAIRY PRODUCTS (Cheese, Churpi, Curd etc.)FERMENTED VEGETABLE PRODUCTS (Gundruk, Sinke, Pickles etc.)BREWERY TECHNOLOGY(Raksi, Jand, Tomba etc.)PLANT BREEDING
3 MODERN BIOTECHNOLOGY MUSHROOM PRODUCTION TISSUE CULTURE BIOFERTILIZERS BIOPROSPECTINGBIOLOGICAL CONTROLVACCINE PRODUCTION
4 PLANT TISSUE CULTURE GOVERNMENT SECTOR Department of Plant Resources Thapathaliproduced 100,000 plantlets of disease free Banana & Citrus.Tissue culturing of Rauwolfia, Eucalyptus & Jerbera, Swertia, Dalbergia etcGodawariPioneer Tissue Culture Labresearch & development; > 100 protocols for plant tissue culture so farIn situ and ex situ conservation of germplasm of improtant medicinal plants
5 National Agricultural Research Council Potato tissue culture lab producing 200,000 virus free pre-basic potato seeds/yearAgricultural Botany Division has initiated anther culture of rice and wheat & also germplasm conservation and diagnostic facilities using PCR technologyDhankuta & Lumle Agriculture Stations also have tissue culture lab facilities
6 Horticulture Development Project of Department of Agriculture, Kirtipur very good facilities for plant tissue culture work (Japanese Grant), once produced in vitro plantlets of apple rootstocksInstitute of Agriculture & Animal Sciences, Rampurplant tissue culture lab facilities (Rockfeller Foundation) but is limited to MSc thesis work only
7 PRIVATE SECTORS Botanical Enterprises Pvt. Ltd., Godawari Nepal Biotech Nursery, BhainsepatiResearch Laboratory for Agriculture Biotechnology & Biochemistry (RLABB), BalkhuMicroplants, Kamal PokhariGreen Research & Technology (GREAT), New BaneshworHimalayan Botanical Research Centre (HIMBORCE), Baneshwor
8 BIOFERTILIZERSDivision of Soil Science & Agriculture Chemistry, Nepal Agricultural Research Council (NARC), Khumaltar: Commercial production of microbial inoculants like rhizobia, Azotobacter.Royal Nepal Academy of Science & Technology (RONAST), Khumaltar: Research on microbial inoculants like rhizobia, Azotobacter, Endo & Ectomycorrhiza, composting, vermicomposting
9 MUSHROOM PRODUCTIONNepal Agricultural Research Institute (NARI), Plant Pathology Division, Khumaltar: spawn for button mushroom, research to develop better substrate for better mushroom production and providing training on mushroom cultivationAgriculture Technology Centre, Gwarko producing spawn for Agaricus bisporus, Sitake (Lentinus edodes), GyanodermaMany unregistered private companies producing mushrooms and spawnRONAST: study of mushroom diversity in Sagarmatha National Park (CNR)DPR: Collection and identification of Nepalese mushrooms around Godawari
10 BIOLOGICAL CONTROL RONAST has been doing research on BIOPESTICIDES specially of plant origin (Eupatorium adenoforum, Lantana camara, Acorus calamus & Ageratum conyzoides) in collaboration with Department of Agriculture against AphidsBIOINSECTICIDESindigenious strains of Bacillus thuriengensisBIOFUNGICIDEScompost & vermicompost tea solution on Foot rot disease of rice and use of above pesticidal plants against club root disease of cauliflower
11 BIOPROSPECTINGRONAST has been doing bioprospecting of Himalayan conifers, Cordyceps sinensis (Yarchagumba) and Taxus bacatta for their antitumor compounds.DPR: phytochemical screening of 12 plant spp. & their antifertility and antihelminthic potential and toxicity test on miceTU Chemistry Division: MSc research workSimhadurbar Baidhya Khana: Crude form of bioprospecting for ayurvedic medicine
12 ADVANCED MODERN BIOTECHNOLOGY RONASTMOLECULAR DIAGNOSTICS (HLB CITRUS DISEASES)MOLECULAR CHARACTERIZATIONS OF:Bacillus thuriengensisMedicinal plants like SwertiaTea clonesGMOs
13 NARCGenetic diversity analysis of isoenzymes in rice, barley, buckwheat, pigeon pea, taro, cucumber, Sweria, Citrus.Application of molecular markers towards development of varities and hybrid in maizeRECASTSelection of genotypes of Mulberry & exploitation of genetic yield potential of common Buckwheat ecotypes.
14 ANIMAL BIOTECHNOLOGYCentral Veterinary Laboratory, Tripureshwor producing vaccine against P.P.R (Pestes des petites Ruminant) disease using tissue culture, rabies vaccine with Japanese grant also exporting to other countries, poultry vaccine, PCR used in disease diagnostic.NARC has been doing animal breeding, embryo transfer and artificial insemination in cattles.
15 MEDICAL BIOTECHNOLOGY The Leprosy Mission, Nepal: determination of cytokines by ELISA for blood immune response studies in leprosy patients & DNA diagnosis of Mycobacterium leprae by PCREverest Biotech Pvt. Ltd.: production of specific antibody required for Human Genome Project, England.TU Teaching Hospital, Maharajgunj: had used PCR for TB bacteria research work
16 TU Teaching Hospital: 3 PCRs mainly used for detecting respiratory viruses for the research purpose onlyKathmandu University Medical School: Korean visiting scientist once tried to identify AIDS virus by using RT PCR but because of the problem in water the project could not be initiated.
18 MANPOWER DEVELOPMENTBachelors level (BSc) : Tribhuvan University, Kathmandu University and many private campuses have been delivering BSc courses in Biotechnology.Masters level (MSc) : Tribhuvan University is going to open MSc courses in Biotechnology from middle of 2006NOTE : National Planning Commision has provided $ 2,00,000 to KU for BSc & $ 70,000 to TU for MSc programmes.
19 BUDGET (RONAST) Fiscal year Total S & T budget Biotechnology budget 2002/2003$120,000(INRA-$21,000; SETS - $70,000)$26,0002003/2004$31,000$6,0002004/2005$44,000$ 23,000(CNR & allowances)2005/2006$28,000$ 12,000
20 BUDGET OF OTHER INSTITUTIONS Budget per yearDivision of Agriculture Botany, NARC, Khumaltar$15,000Biotechnology Laboratory of Department of Plant Resources, Thapathali and Godawari$10,000Phytochemical and microbial screening at DPR
21 POTENTIALS OF MODERN BIOTECHNOLOGIES Genetic material of one sp. can be inserted into another, crossing natural barriers that were previously impossible.BETTER CROPSRESISTANT TO INSECTSRESISTANT TO DISEASESEVEN TO NATURAL CALAMITIES LIKE DROUGHT
22 SOLUTIONS TO THE THREAT OF HUNGER BY HELPING TO INCREASE FOOD PRODUCTION & MALNUTRITION BY CREATING RICE THAT PROVIDES VITAMIN A & IRONMAKING AGRICULTURE MORE ENVIRONMENTALLY SUSTAINABLE THROUGH PEST RESISTANT CROPS
23 BIOPROSPECTINGResearch for new chemicals in living things that will have some medical or commercial usea high risk area for investors but can have massive returnsOf the world's 25 top-selling pharmaceuticals, 10 originally sourced from animals, plants or micro-organisms (in 1995, these accounted for nearly $US14 billion in global sales )Pharmaceutical companies and agribusiness use indigenous knowledge as a precursor to screening, and this is happening with little regard for the protection of indigenous intellectual property and with no equitable sharing of profits
24 CHALLENGES OF MODERN BIOTECHNOLOGY HEALTH RISKS POISED BY GMO CROPSPOTENTIAL ENVIRONMETAL IMPACTS OF THE RELEASE OF GMOS LEADING TO SERIOUS CONSEQUENCES FOR THE BIODIVERSITY THAT MANY COMMUNITIES RELY ON FOR THEIR FOOD, LIVELIHOODS AND CULTURAL SURVIVAL
25 BIOTECHNOLOGY & BIOSAFETY POLICIES Ministry of Environment, Science & Technology had submitted a Biotechnology Policy to the cabinet four years ago for the Govt approval but has not yet been approved.The Ministry of Forest & Soil Conservation aims to develop & formulate the Biosafety Policy, legal & administrative framework to safeguard the biological diversity, human health and environment from the adverse effects of GMOs & their products in accord with the CBD CPB.
26 PROBLEMSLACK OF QUALIFIED MANPOWER & BRAIN DRAINLACK OF GOVERNMENT POLICIES, RULES & REGULATIONSLACK OF ADEQUATE FUNDINGSLACK OF PROPER INFRASTRUCTURESFAILURE TO IMPLIMENT RECOMMENDATIONS PROPOSED BY VARIOUS TASK FORCESLACK OF INVOLVEMENT OF PRIVATE SECTOR AND TEACHING INSTITUTIONS
27 THE APPLICATION OF GENETIC ENGINEERING TECHNIQUES TO THE DEVELOPMENT OF NEW DRUGS AND VACCINES FOR DEVELOPING COUNTRY LIKE OURS IS AN EXPENSIVE AND LONG TERM INVESTMENT
28 IMMEDIATE ATTENTIONSTRENGTHENING OF EXISTING INFRASTRUCTURES AT DIFFERENT INSTITUTIONSMANPOWER DEVELOPMENTRESEARCH & DEVELOPMENT PROJECTS IN COLLABORATION WITH OTHER COUNTRIES