1. Histology for Pathology Hematopoietic Elements
Theresa Kristopaitis, MD
Associate Professor
Director of Mechanisms of Human Disease
Kelli A. Hutchens, MD, FCAP
Assistant Professor
Assistant Director of Mechanisms of Human Disease
Loyola Stritch School of Medicine
Welcome to basic histology of the skin

2. Objectives
On a peripheral blood smear identify the following cells: erythrocytes, platelets, neutrophils, eosinophils, basophils, lymphocytes and monocytes.
On a peripheral blood smear identify neutrophil band cells (also known as stab cells) and state the clinical significance of their presence.
On H&E stained sections, identify neutrophils, eosinophils, lymphocytes.
On H&E sections identify plasma cells.
On a stained section identify the 3 major components of bone marrow (bone trabeculae, hematopoietic cells, adipose tissue).
List the sequence of development of a granulocytes – focusing on the neutrophil.
On a section of bone marrow, identify a megakaryocyte.
Define “blast cell”.

3. Granulocytes – Peripheral Blood Smear
Neutrophil
Eosinophil
Basophil

4. Agranulocytes – Peripheral Blood Smear
Lymphocyte
Monocyte

5. Peripheral Blood Smear
Red blood cells
Platelets

6. H&E Stained Sections
Eosinophils
Neutrophils

7. H&E Stained Section

8. H&E Section – Plasma Cells

9. Bone Marrow
Bone
Hematopoietic
cells
Adipose (fat) cells

10. Based on this representative section of normal bone marrow, what is the age the patient?

11. Granulocytopoiesis A B C E G F Neutrophilic series A = Myeloblast
B = Myelocyte (large cell, rounded nucleus)
C = Late myelocyte or early metamyelocyte (nucleus beginning to indent)
E = Metamyelocyte (indented nucleus)
G = Band cell (much thinner nucleus)
F = Segmented (mature) neutrophil

12. Megakaryocyte (Mega = Giant) Fragments of cytoplasm break off to become platelets