Presentation is loading. Please wait.

Presentation is loading. Please wait.

Hematopoeisis VIBS 443/602. Peripheral blood smear (May-Grunwald-Giemsa) 113.

Similar presentations


Presentation on theme: "Hematopoeisis VIBS 443/602. Peripheral blood smear (May-Grunwald-Giemsa) 113."— Presentation transcript:

1 Hematopoeisis VIBS 443/602

2 Peripheral blood smear (May-Grunwald-Giemsa) 113

3 Peripheral blood smear (Leishman-Giemsa) eosinophil, basophil, and neutrophils 110

4 Bone marrow

5

6

7 Bone marrow smear; low power view, showing negative images of fat globules

8 Megakaryocyte: contain many joined nuclei. Each probably contain 8-32 times a normal diploid amount of DNA 1.Nuclei of megakaryocyte 2.Red blood cell

9 Macrophage: contains greenish, iron-containing granules due to poor quality. Differentiating cells have been torn away during preparation. 1.Macrophage 2.Lymphocyte 3.Neutrophil

10 1.Pronormoblast 2.Basophilic normoblast 3.Polychromatophilic normoblast 4.Orthochromatic normoblast 5.Myelocyte 6.Late metamyelocytes

11 1.Pronormoblast 2.Basophilic normoblast 3.Polychromatophilic normoblast 4.Orthochromatic normoblast 5.Myelocyte 6.Late metamyelocytes

12 1.Late promyelocyte 2.Late myelocyte/early metamyelocyte 3.Nearly mature PMN

13 1.Smudge cell 2.Promyelocyte 3.Myelocyte 4.Metamyelocytes

14 1.Pronormoblast 2.Metamyelocytes 3.Band cell

15 Bone marrow: EM 12a Reticular cell Developing red blood cell

16 Bone marrow; EM 8a 1.Lymphocyte 2.PNM 3.Red blood cell 4.eosinophil

17 Bone marrow; EM 12b 1.PMN 2.Immature neutrophil 3.Red blood cell

18 Routine H&E of bone Marrow spicules Lipid in fat cells

19 H&E of bone marrow 1. megakaryocytes

20 19761

21 H&E of bone marrow: Red blood cell

22 Rib 420

23 Rib 420

24 Tibia, fetal 421

25 32583

26

27 Red bone marrow smear (May-Grunwald-Giemsa) 112

28

29

30 Pronormoblast round nucleus and thin rim of deep blue cytoplasm Basophilic normoblast nuclear condensation ("fractured nucleus") with a deep blue cytoplasm Cells of Erythropoisesis

31 Polychromatophilic normoblast cytoplasm has accumulated enough hemoglobin so that it is no longer blue but a bluish-gray to bluish-red Orthochromatic normoblast must have an almost mature cytoplasm with only the faintest hint of blue: the nucleus is fully condensed

32 Reticulocyte no nucleus, mature cytoplasm with blue tint Red blood cell typical of mature cell seen in circulation

33

34

35 Myelocyte cytoplasm becomes increasingly mature (reddish/clear) but still contains blue, nucleus of variable shape Cells of Granulopoiesis Promyelocyte oval nucleus with deep blue cytoplasm and azurophilic granules

36 Metamyelocyte deeply indented nucleus with nearly mature cytoplasm (no longer blue) Band cell nucleus just prior to lobulation with fully mature cytoplasm Neutrophil (mature) lobulated nucleus with fully mature cytoplasm

37

38

39

40


Download ppt "Hematopoeisis VIBS 443/602. Peripheral blood smear (May-Grunwald-Giemsa) 113."

Similar presentations


Ads by Google