Granulocytes: Neutrophils Most numerous WBC in blood Multilobed nucleus Granules: –Azurophilic granules –Specific granules Function –1 st wave of cells in acute inflammation; can phagocytose bacteria
Granulocytes: Basophils Rare! Lobulated nucleus often obscured by granules Dark Blue Granules –Hydrolytic enzymes, heparin sulfate, histamine, SRS Function –Role in hypersensitivity and anaphylaxis
Granulocytes: Eosinophils Bilobed nucleus Bright pink Granules –Arginine rich major basic protein, peroxidase, histaminase, arylsulfatase Function: –Important in allergic rxns, parasitic infections, and phagocytosis of Ab-Ag complexes
Agranulocytes: Monocytes Largest WBCs in blood smear Migrate through blood to the tissues; once in tissues they differentiate into phagocytes (macrophages)
Agranulocytes: Lymphocytes About size of RBCs Function –Main functional cells of adaptive immune system –T cells –B cells
Questions Which of the following cell types would be expected to be increased in number with a bacterial infection? Viral? Parasitic?
Polychromatophilic Erythroblast Smaller than basophilic erythroblast Smaller intensely heterochromatic nucleus Purple/lilac cytoplasm mix of basophilia from ribosomes and growing eosinophilia from hemoglobin LAST MITOTIC STAGE!
Normoblast Smaller than polychromatophilic erythroblast Small, compact, intensely staining nucleus that is getting ready to be extruded Eosinophilic cytoplasm due to abundant hemoglobin
Reticulocyte Immature RBC that has polyribosomes Appear as polychrom- atophilic erythrocyte on blood smear When stained with a special (supravital) stain Reticulocyte
Erythrocyte Smallest Eosinophilic due to hemoglobin NO NUCLEUS!
What are the Granulocytes? These are cells of the immune system (White Blood Cells) that contain vesicles. The vesicle contents vary among cell lines and stain differently. Neutrophils (polymorphonuclear or PMN cells) Eosinophils Basophils
Trend of Development Larger to smaller Azure granules to specific granules Round nucleus to altered shape nucleus Granulocyte Lineage: Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Granulocyte (Erythroid: Proerythroblast →Basophilic erythroblast → Polychromatophilic erythroblast → Normoblast → Reticulocyte → Erythrocyte)