18Polychromatophilic Erythroblast Smaller than basophilic erythroblastSmaller intensely heterochromatic nucleusPurple/lilac cytoplasm mix of basophilia from ribosomes and growing eosinophilia from hemoglobinLAST MITOTIC STAGE!
19Normoblast Smaller than polychromatophilic erythroblast Small, compact, intensely staining nucleus that is getting ready to be extrudedEosinophilic cytoplasm due to abundant hemoglobin
20Reticulocyte Immature RBC that has polyribosomes Appear as polychrom-atophilic erythrocyte on blood smearWhen stained with a special (supravital) stain Reticulocyte
21ErythrocyteSmallestEosinophilic due to hemoglobinNO NUCLEUS!
25What are the Granulocytes? These are cells of the immune system (White Blood Cells) that contain vesicles. The vesicle contents vary among cell lines and stain differently.Neutrophils (polymorphonuclear or PMN cells)EosinophilsBasophils
26Trend of Development Larger to smaller Azure granules to specific granulesRound nucleus to altered shape nucleusGranulocyte Lineage:Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Granulocyte(Erythroid: Proerythroblast →Basophilic erythroblast → Polychromatophilicerythroblast → Normoblast → Reticulocyte → Erythrocyte)