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Blood and Bone Marrow Histology SSN November 18, 2004 Presented by: Nadia Goodwin & Missy Walker.

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Presentation on theme: "Blood and Bone Marrow Histology SSN November 18, 2004 Presented by: Nadia Goodwin & Missy Walker."— Presentation transcript:

1 Blood and Bone Marrow Histology SSN November 18, 2004 Presented by: Nadia Goodwin & Missy Walker

2 Formed Elements of the Blood

3 Peripheral Blood: Erythrocytes RBCs constitute the largest number of cells in the blood Biconcave discs NO NUCLEUS Contain Hemoglobin

4 Peripheral Blood: Platelets Derived from Megakaryocytes in bone marrow – formed from small bits of Megakaryocyte cytoplasm Function in blood clotting

5 Peripheral Blood: Leukocytes GRANULOCYTES –Neutrophils –Basophils –Eosinophils AGRANULOCYTES –Lymphocytes (T and B cells) –Monocytes (Macrophages)

6 Peripheral Blood: Leukocytes GRANULOCYTES –Neutrophils –Basophils –Eosinophils

7 Granulocytes: Neutrophils Most numerous WBC in blood Multilobed nucleus Granules: –Azurophilic granules –Specific granules Function –1 st wave of cells in acute inflammation; can phagocytose bacteria

8 Granulocytes: Basophils Rare! Lobulated nucleus often obscured by granules Dark Blue Granules –Hydrolytic enzymes, heparin sulfate, histamine, SRS Function –Role in hypersensitivity and anaphylaxis

9 Granulocytes: Eosinophils Bilobed nucleus Bright pink Granules –Arginine rich major basic protein, peroxidase, histaminase, arylsulfatase Function: –Important in allergic rxns, parasitic infections, and phagocytosis of Ab-Ag complexes

10 Agranulocytes: Monocytes Largest WBCs in blood smear Migrate through blood to the tissues; once in tissues they differentiate into phagocytes (macrophages)

11 Agranulocytes: Lymphocytes About size of RBCs Function –Main functional cells of adaptive immune system –T cells –B cells

12 Questions Which of the following cell types would be expected to be increased in number with a bacterial infection? Viral? Parasitic?


14 RBC Development Watch for trends!

15 Proerythroblast Biggest in lineage Large central nucleus with one or two nucleoli Basophilic cytoplasm b/c ribosomes Look for Golgi ghost

16 Basophilic Erythroblast Smaller than proerythroblast Checkerboard nucleus (heterochromatic) Intense basophilia (lots of ribosomes!)

17 Proerythroblast vs Basophilic Erythroblast

18 Polychromatophilic Erythroblast Smaller than basophilic erythroblast Smaller intensely heterochromatic nucleus Purple/lilac cytoplasm mix of basophilia from ribosomes and growing eosinophilia from hemoglobin LAST MITOTIC STAGE!

19 Normoblast Smaller than polychromatophilic erythroblast Small, compact, intensely staining nucleus that is getting ready to be extruded Eosinophilic cytoplasm due to abundant hemoglobin

20 Reticulocyte Immature RBC that has polyribosomes Appear as polychrom- atophilic erythrocyte on blood smear When stained with a special (supravital) stain  Reticulocyte

21 Erythrocyte Smallest Eosinophilic due to hemoglobin NO NUCLEUS!

22 Erythropoiesis


24 Development of Granulocytes

25 What are the Granulocytes? These are cells of the immune system (White Blood Cells) that contain vesicles. The vesicle contents vary among cell lines and stain differently. Neutrophils (polymorphonuclear or PMN cells) Eosinophils Basophils

26 Trend of Development Larger to smaller Azure granules to specific granules Round nucleus to altered shape nucleus Granulocyte Lineage: Myeloblast → Promyelocyte → Myelocyte → Metamyelocyte → Band → Granulocyte (Erythroid: Proerythroblast →Basophilic erythroblast → Polychromatophilic erythroblast → Normoblast → Reticulocyte → Erythrocyte)

27 Myeloblast Prominent nucleoli

28 PROmyelocyte Details Golgi ghost Can’t distinguish type of granulocyte yet Eccentric nucleus Don’t confuse with proerythroblast or basophilic erythroblasts

29 Myelocyte Last stage in which mitosis can occur Begin to see who’s a neutrophil, who’s an eosinophil (who’s a basophil) Golgi ghost

30 Neutrophilic Myelocyte vs Eosinophilic Myelocyte

31 METAmyelocyte nucleus changing shape, not spherical Soooo... No more mitosis

32 Band Cell Immature granulocyte Elongated nucleus of nearly uniform width; horseshoe/U-shaped

33 Finally...

34 Myeloblast – no granules capable of mitosis Promyelocyte – azurophilic granules Mylocyte – specific granules Metamylocyte – specifc granules nucleus changes shape Band cell – (PMN only) specific granules Mature Granulocyte – specific granules

35 Compare what you see...

36 Megakaryocyte Huge Platelets form from invaginations of this Multilobular nucleus

37 Megakaryocyte


39 Don’t Worry, Be Happy!

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