1. Copyright © 2004 Pearson Education, Inc., publishing as Benjamin Cummings Fundamentals of Anatomy & Physiology SIXTH EDITION Frederic H. Martini Lecture 19, Blood Lecturer: Dr. Ebadi Room P313 Phone: (718) 260-5285 E-Mail: ibarjis@citytech.cuny.edu

2. Learning Objectives List the components of the cardiovascular system and explain the major functions of this system. Describe the important components and major functions of the blood List the characteristics and functions of red blood cells. Describe the structure of hemoglobin and indicate its functions. Discuss red blood cell production and maturation.

3. Learning Objectives Explain the importance of blood typing and the basis for ABO and Rh incompatibilities. Categorize the various white blood cells on the basis of structure and function. Describe the structure, function and production of platelets. Describe the reaction sequences responsible for blood clotting.

4. Provides a mechanism for rapid transport of nutrients, waste products, respiratory gases and cells The cardiovascular system

5. Fluid connective tissue Functions include Transporting dissolved gases, nutrients, hormones, and metabolic wastes Regulating pH and ion composition of interstitial fluids Restricting fluid loss at injury sites Defending the body against toxins and pathogens Regulating body temperature by absorbing and redistributing heat Functions and Composition of Blood

6. Blood Composition

7. The composition of blood Plasma and formed elements comprise whole blood. Plasma elements include blood cells: Red blood cells (RBC) White blood cells (WBC) Platelets Can fractionate whole blood for analytical or clinical purposes

8. The Composition of Whole Blood Fresh whole blood for testing in a lab is usually collected from a superficial vein. When checking the efficiency of gas exchange, it may be necessary to draw a blood sample from an artery

9. The Composition of Whole Blood The chief difference between plasma and interstitial fluid involves the concentration of dissolved oxygen and proteins.

10. The Composition of Whole Blood

11. Process of blood cell formation Hemocytoblasts are circulating stem cells that divide to form all types of blood cells Whole blood from anywhere in the body has roughly the same temperature ( 38ºC ), pH (7.4) and viscosity. Bright red color if taken from artery Dull red color if taken from vein Hemopoiesis

12. Accounts for 46-63% of blood volume 92% of plasma is water Higher concentration of dissolved oxygen and dissolved proteins than interstitial fluid Plasma

13. more than 90% are synthesized in the liver Albumins are the most abundant plasma proteins 60% of plasma proteins Responsible for viscosity and osmotic pressure of blood Plasma proteins

14. Globulins ~35% of plasma proteins Include immunoglobins which attack foreign proteins and pathogens Include transport globulins which bind ions, hormones and other compounds Fibrinogen Converted to fibrin during clotting Are necessary for blood clotting Removal of fibrinogen leaves serum Additional Plasma Proteins

15. Erythrocytes (RBC) account for slightly less than half the blood volume, and 99.9% of the formed elements. Hematocrit measures the percentage of whole blood occupied by formed elements Commonly referred to as the volume of packed red cells Red Blood Cells Abundance of RBCs

16. Biconcave disc, providing a large surface to volume ration Shape allows RBCs to stack, bend and flex RBCs lack organelles Typically degenerate in about 120 days. Structure of RBCs

17. The Anatomy of Red Blood Cells

18. Molecules of hemoglobin account for 95% of the proteins in RBCs Hemoglobin is a globular protein, formed from two pairs of polypeptide subunits Each subunit contains a molecule of heme which reversibly binds an oxygen molecule Damaged or dead RBCs are recycled by phagocytes Hemoglobin

19. The Structure of Hemoglobin

20. “Sickling” in Red Blood Cells

21. Replaced at a rate of approximately 3 million new blood cells entering the circulation per second. Replaced before they hemolyze Components of hemoglobin individually recycled Heme stripped of iron and converted to biliverdin, then bilirubin Iron is recycled by being stored in phagocytes, or transported throughout the blood stream bound to transferrin RBC life span and circulation

22. Red Blood Cell Turnover

23. Erythropoeisis = the formation of new red blood cells Occurs in red bone marrow Process speeds up with in the presence of EPO (Erythropoeisis stimulating hormone) RBCs pass through reticulocyte and erythroblast stages RBC Production

24. Stages of RBC Maturation

25. Determined by the presence or absence of surface antigens (agglutinogens) Antigens A, B and Rh (D) Antibodies in the plasma (agglutinins) Cross-reactions occur when antigens meet antibodies Blood types

26. Blood Typing and Cross-Reactions

27. Blood Type Testing

28. Rh Factors and Pregnancy

29. Have nuclei and other organelles Defend the body against pathogens Remove toxins, wastes, and abnormal or damaged cells Are capable of amoeboid movement (margination) and positive chemotaxis Some are capable of phagocytosis The White Blood Cells Leukocytes

30. Granular and agranular Granular leukocytes Neutrophils – 50 to 70 % total WBC population Eosinophils – phagocytes attracted to foreign compounds that have reacted with antibodies Basophils – migrate to damaged tissue and release histamine and heparin Types of WBC

31. Agranular leukocytes Agranular leukocytes are formed inred bone marrow. Agranular leukocytes include: Monocytes - become macrophage Lymphocytes – includes T cells, B cells, and NK cells Types of WBC

32. White Blood Cells

33. Indicates a number of disorders Leukemia = inordinate number of leukocytes Differential count

34. Granulocytes and monocytes are produced by bone marrow stem cells Divide to create progenitor cells Stem cells may originate in bone marrow and migrate to peripheral tissues Several colony stimulating factors are involved in regulation and control of production WBC Production

35. Figure 19.12 The Origins and Differentiation of Formed Elements Animation: The origins and differentiation of blood cells (see tutorial)

36. Flattened discs Circulate for 9-12 days before being removed by phagocytes Platelets

37. Transporting chemicals important to clotting Forming temporary patch in walls of damaged blood vessels Contracting after a clot has formed Platelet functions

38. Megakaryocytes release platelets into circulating blood Rate of platelet formation is stimulated by thrombopoietin, thrombocyte-stimulating factor, interleukin-6, and Multi-CSF Platelet production (thrombocytopoiesis)

39. Hemostasis Prevents the loss of blood through vessel walls Three phases – Vascular phase Platelet phase Coagulation phase

40. Hemostasis Vascular phase Local blood vessel constriction (vascular spasm) Platelet phase Platelets are activated, aggregate at the site, adhere to the damaged surfaces

41. The Vascular and Platelet Phases of Hemostasis

42. Coagulation phase Factors released by platelets and endothelial cells interact with clotting factors to form a clot Extrinsic pathway Intrinsic pathway Common pathway Suspended fibrinogen is converted to large insoluble fibrin fibers

43. The Coagulation Phase of Hemostasis

45. Clot retraction Final phase of healing Platelets contract and pull the edges of the vessel together

46. Fibrinolysis Clot gradually dissolves through action of plasmin Activated form of plasminogen Clotting can be prevented through the use of drugs that depress the clotting response or dissolve existing clots Anticoagulants include heparin, coumadin, aspirin, dicumarol, t- PA, streptokinase, and urokinase

47. You should now be familiar with: The components of the cardiovascular system and its major functions. The important components and major functions of the blood. The characteristics and functions of red blood cells. The structure of hemoglobin and its functions. Red blood cell production and maturation.

48. You should now be familiar with: The importance of blood typing and the basis for ABO and Rh incompatibilities. The various white blood cells. The structure, function and production of platelets. The reaction sequences responsible for blood clotting.