1. Creatine supplementation prevents fatty liver in rats fed choline-deficient diet: a burden of one-carbon and fatty acid metabolism 
Rafael Deminice, Gabriela Salim Ferreira de Castro, Lucas Vieira Francisco, Lilian Eslaine Costa Mendes da Silva, João Felipe Rito Cardoso, Fernando Tadeu Trevisan Frajacomo, Bruno Gonzaga Teodoro, Leonardo dos Reis Silveira, Alceu Afonso Jordao 
Journal of Nutritional Biochemistry 
Volume 26, Issue 4, Pages (April 2015)
DOI: /j.jnutbio
Copyright © 2015 Elsevier Inc. Terms and Conditions

2. Fig. 1
Liver total fat (A), cholesterol (B), triglycerides (C) and photomicrography of liver sections stained with H&E (100×) of rats fed with a C, CDD or CR diets for 4 weeks. Rats fed with CDD show macrovesicular fat infiltration (black arrows) which was reverted with CR. Values are mean±standard error, n=8. a,b Labeled means without a common letter differ (P<0.05; ANOVA with posttest of Tukey).
Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio )
Copyright © 2015 Elsevier Inc. Terms and Conditions

3. Fig. 2
Creatine, Hcy and PC metabolic interactions (A); methionine metabolism (B); phospholipids metabolism (C); and MTP (D) genes mRNA of rats fed with a C, CDD or CR diets for 4 weeks. Values are mean±standard error, n=8. a,b Labeled means without a common letter differ (P<0.05; ANOVA with posttest of Tukey). CDD impaired ChDh and BHMT gene expression which results in decreased hepatic SAM availability and increased Hcy. CR reversed BHMT and GNMT egen expression, however, was unable to increase hepatica SAM concentration. AGAT, arginine:glycine amidinotransferase; GAMT, SAM:guanidinoacetate N-methyltransferase; PE, phosphatidlyethanolamine; CT, CTP:phosphorylcholine cytidylyltransferase; BHMT, betaine-homocysteine S-methyltransferase; GNMT, Glycine N-methyltransferase.
Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio )
Copyright © 2015 Elsevier Inc. Terms and Conditions

4. Fig. 3
Hepatic transcription factors (A); fatty acid oxidation genes mRNA (B); and PPARγ protein expression of rats fed with a C, CDD or CR diets for 4 weeks. Values are mean±standard error, n=8. a,b Labeled means without a common letter differ (P<0.05; ANOVA with posttest of Tukey). CDD modulated substantially transcription factors and key genes related to fatty acid b-oxidation pathway, which was reversed by CR.
Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio )
Copyright © 2015 Elsevier Inc. Terms and Conditions