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Venkata J. Adapala, Sunday A. Adedokun, Robert V. Considine, Kolapo M

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Presentation on theme: "Venkata J. Adapala, Sunday A. Adedokun, Robert V. Considine, Kolapo M"— Presentation transcript:

1 Acute inflammation plays a limited role in the regulation of adipose tissue COL1A1 protein abundance 
Venkata J. Adapala, Sunday A. Adedokun, Robert V. Considine, Kolapo M. Ajuwon  Journal of Nutritional Biochemistry  Volume 23, Issue 6, Pages (June 2012) DOI: /j.jnutbio Copyright © 2012 Elsevier Inc. Terms and Conditions

2 Fig. 1 Expression of selected inflammatory genes in subcutaneous (SWAT) or epididymal (EWAT) adipose tissue of mice under HFD or acute inflammation (LPS). Expression of TGFB1 (A and B), IL6 (C and D) and TNFα (E and F) was determined in both SWAT and EWAT. Bars represent means±S.E. Bars with different superscript letters are different (P<.05). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2012 Elsevier Inc. Terms and Conditions

3 Fig. 2 Gene expression analysis of selected ECM genes in subcutaneous (SWAT) or epididymal (EWAT) adipose tissue of mice under HFD or acute inflammation. Gene expression analysis of BGN (A and B), COL1A1 (C and D) and COL6A1 (E and F) were determined in both SWAT and EWAT. Expression of individual genes was normalized to 18s with the ΔΔCt method. Bars represent means±S.E. Bars with different superscript letters are different (P<.05). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2012 Elsevier Inc. Terms and Conditions

4 Fig. 3 Effect of HFD and acute inflammation on protein abundance of BGN and COL1A1 in epididymal tissue. Densitometric data were obtained for the abundance of BGN and COL1A1 and normalized to β-actin. (Fig. 3B and C). Bars represent means±S.E.M. Bars with different superscript letters are different (P<.05). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2012 Elsevier Inc. Terms and Conditions

5 Fig. 4 Effect of obesity on COL1A1 expression in human subcutaneous adipose tissue. Subcutaneous adipose tissue was obtained from lean (Lean-Sc) or obese (Obese-Sc) human subjects. COL1A1 mRNA abundance was determined by RT-PCR (Fig. 4A). Protein abundance is normalized to β-actin (B and C). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2012 Elsevier Inc. Terms and Conditions

6 Fig. 5 Effect of high-fat (HF) diet and acute inflammation with LPS on MMP expression. Expression of MMPs was determined by RT-PCR and normalized to 18s. Bars with different superscript letters are different (P<.05). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2012 Elsevier Inc. Terms and Conditions

7 Fig. 6 Effect of HFD and obesity on MMP activity. Matrix metalloprotease activity was determined with gelatin zymography. Zymography was performed on epididymal adipose tissue from control (CON) and HFD-fed mice given either saline (CON-SAL, CON-LPS, HF-SAL and HF-LPS) (A) and subcutaneous human subcutaneous adipose tissue from lean (Lean-Sc) or obese (Obese-Sc) human subjects (B). Increased MMP9 activities were found in A and B. Bars with different superscript letters are different (P<.05). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2012 Elsevier Inc. Terms and Conditions

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