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High dietary salt decreases antioxidant defenses in the liver of fructose-fed insulin- resistant rats  Waleska Claudia Dornas, Wanderson Geraldo de Lima,

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Presentation on theme: "High dietary salt decreases antioxidant defenses in the liver of fructose-fed insulin- resistant rats  Waleska Claudia Dornas, Wanderson Geraldo de Lima,"— Presentation transcript:

1 High dietary salt decreases antioxidant defenses in the liver of fructose-fed insulin- resistant rats  Waleska Claudia Dornas, Wanderson Geraldo de Lima, Rinaldo Cardoso dos Santos, Joyce Ferreira da Costa Guerra, Melina Oliveira de Souza, Maísa Silva, Lorena Souza e Silva, Mirla Fiuza Diniz, Marcelo Eustáquio Silva  Journal of Nutritional Biochemistry  Volume 24, Issue 12, Pages (December 2013) DOI: /j.jnutbio Copyright © 2013 Elsevier Inc. Terms and Conditions

2 Fig. 1 Food, fluid, caloric intake and serum leptin of experimental rats. Symbols represent the animals in scatter plots to each group. Different letters indicate significant differences at P<.05 by one-way ANOVA followed by Tukey’s test. Control diet (CON); high-salt diet (HS); high-fructose diet (HF); high-fructose and salt (HFS) diet for 10 weeks after previous treatment for a period of 10 weeks with HF. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2013 Elsevier Inc. Terms and Conditions

3 Fig. 2 (A-D) Representative photomicrographs H&E staining of liver sections of experimental rats. Hepatocytes abnormalities were not observed in the CON group (A); note hyperemic vessels (arrowhead) and normal parenchyma appearance in the HS group (B); aspect of macrovesicular steatosis in the HF group (C). Note hepatocytes with a large negative image in the cytoplasm with nucleus displaced into the periphery of the cell, large fat globule in most cases (white arrow); aspect of microvesicular steatosis in the HFS group (D). Note cells with small cytoplasmic vacuoles without displaced nucleus (black arrow) magnification ×440. Grade of liver steatosis of rats in experimental groups (E). Values (medianne, n=8–11) (*P<.05 vs. control rats). Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2013 Elsevier Inc. Terms and Conditions

4 Fig. 3 Antioxidant defenses and oxidative stress in the liver of experimental rats. Different letters indicate significant differences at P<.05 by one-way ANOVA followed by Tukey’s test. Journal of Nutritional Biochemistry  , DOI: ( /j.jnutbio ) Copyright © 2013 Elsevier Inc. Terms and Conditions

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