3.What is the principle behind the Quantiferon-Tb assay for TB?

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Presentation transcript:

3.What is the principle behind the Quantiferon-Tb assay for TB?

What is the principle behind the Quantiferon-Tb assay for TB? Definition: It is a whole-blood test for use as an aid in diagnosing Mycobacterium tuberculosis infection – latent tuberculosis infection (LTBI) – tuberculosis (TB) disease

What is the principle behind the Quantiferon-Tb assay for TB? The principle relies on the quantitative (in vitro) release of interferon gamma by the lymphocytes, upon exposure of M. tuberculosis proteins antigens – include mixtures of synthetic peptides : ESAT-6 and CFP-10

Test tube 1 Nil Control tube Whole Blood Cell Incubation at 37°C for hrs Interferon Gamma Measurements by ELISA Test Tube 3 Mitogen Control tube. Test tube 2 TB Antigen tube

Interpretation of Results

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