1. Role of the T-cell interferon-gamma release assays in preventing reactivation of latent tuberculosis infection in immunosuppressed patients in treatment with anti-TNF agents 
Jose Domínguez, Irene Latorre 
Journal of Crohn's and Colitis 
Volume 2, Issue 3, Pages (September 2008)
DOI: /j.crohns
Copyright © 2008 European Crohn's and Colitis Organisation Terms and Conditions

2. Figure 1
T-SPOT.TB detects the IFN-γ produced by peripheral blood isolated mononuclear cells by means of ELISPOT method, after stimulation with ESAT-6 and CFP-10. Subjects are considered positive if there is response to one or both of the specific antigens (A). The result is considered negative when there is not response for any of the specific antigens (B). The presence of reactive antigen-specific T cells is revealed as a spot on the well. Spots can be scored manually or also with the aid of an automated ELISPOT plate reader. Test wells are scored as positive if they contain at least six spot-forming cells more than the negative control well and this number is at least twice the number of the negative control well. The result of the assay is considered indeterminate if the number of spots in the positive control is less than 20, and the response to both of the specific antigens is negative (C).
Journal of Crohn's and Colitis 2008 2, DOI: ( /j.crohns )
Copyright © 2008 European Crohn's and Colitis Organisation Terms and Conditions