1. Diagnostic Performance of a Cytokine and IFN-γ–Induced Chemokine mRNA Assay after Mycobacterium tuberculosis–Specific Antigen Stimulation in Whole Blood from Infected Individuals 
Sunghyun Kim, Hyejon Lee, Hyunjung Kim, Yeun Kim, Jang-Eun Cho, Hyunwoo Jin, Dae Yeon Kim, Sang-Jun Ha, Young Ae Kang, Sang-Nae Cho, Hyeyoung Lee 
The Journal of Molecular Diagnostics 
Volume 17, Issue 1, Pages (January 2015)
DOI: /j.jmoldx
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Target gene mRNA expression levels after stimulation of Mycobacterium tuberculosis (MTB)–specific antigens for 24 hours in all study subjects. Whole blood of study subjects [active pulmonary tuberculosis (PTB) patient, latent tuberculosis infection (LTBI) group, and healthy control group] were stimulated with MTB-specific antigens (early secretory antigen target 6, culture filtrate protein 10, and TB 7.7) for 24 hours and the mRNA expression levels for target genes were analyzed by real-time RT-PCR. A: Th1-type factors: interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-2R. B: Th2-type cytokines: IL-4 and IL-10. C: IFN-γ–induced chemokines: CXCL9, CXCL10, and CXCL11. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.
The Journal of Molecular Diagnostics  , 90-99DOI: ( /j.jmoldx )
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Comparison of receiver operator characteristic (ROC) curve of eight single-target, real-time RT-PCR assays. A: Th1-type factors: Interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-2R. B: Th2-type cytokines: IL-4 and IL-10. C: IFN-γ–induced chemokines: CXCL9, CXCL10, and CXCL11. The P values, 95% CIs, and areas are indicated for each factor. Ag/nil, antigen per nil.
The Journal of Molecular Diagnostics  , 90-99DOI: ( /j.jmoldx )
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
mRNA expression levels of multitarget genes after stimulation of Mycobacterium tuberculosis (MTB)-specific antigens for 24 hours in all study subjects. Whole blood of study subjects [active pulmonary tuberculosis (PTB) patient, latent tuberculosis infection (LTBI) group, and healthy control group] were stimulated with MTB-specific antigens (early secretory antigen target 6, culture filtrate protein 10, and TB 7.7) for 24 hours and simultaneous detection of multitarget genes were analyzed by real-time RT-PCR. A: Sum of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-2R mRNA expression. B: Sum of TNF-α, IL-2R, CXCL9, and CXCL10. ∗∗P < 0.01, ∗∗∗P < 0.001.
The Journal of Molecular Diagnostics  , 90-99DOI: ( /j.jmoldx )
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5. Figure 4
Comparison of ROC curve of multitarget real-time RT-PCR assays. A: Sum of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, and IL-2R mRNA expression. B: Sum of TNF-α, IL-2R, CXCL9, and CXCL10. The P values, 95% CIs, and areas are indicated for each combination. Ag/nil, antigen per nil.
The Journal of Molecular Diagnostics  , 90-99DOI: ( /j.jmoldx )
Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions