Small Talk Cell-to-Cell Communication in Bacteria.

Slides:

Advertisements

Similar presentations

The 12 “Most Unwanted” Bacteria

Advertisements

Lateral Transfer. Donating Genes Mutation often disrupts the function of a gene Gene transfer is a way to give new functions to the recipient cell Thus,

 Mucous membranes –Conjunctiva –Respiratory –Gastrointestinal tract –Urogenital  Skin –Abrasions or bite  Parenteral –Puncture or injection Portals.

For Lecture 3 Exam The whole test is matching. Be able to match the following with their description: Virulence factors/enzymes The three hemolysis patterns.

Bacterial Physiology A Proteomic Approach to Oral Diseases Oral Diseases Peter Zilm Microbiology Laboratory Dental School The University of Adelaide.

 Cefixime is quickly establishing in Western countries as a potent broad-spectrum antibiotic with a variety of indications. A multinational, nonrandomized.

Taxonomy Defined as the science of classification of organisms Taxonomic categories are arranged to show degree of similarities among organisms Relatedness.

Division of Bacterial, Parasitic and Allergenic Products Jay E. Slater, MD Director, DBPAP 25 October 2013.

BioFire (FilmArray) Multiplex PCR Assays

Satellite Workshop: Information Processing in the Biological Organism (A Systems Biology Approach) Fred S. Roberts Rutgers University.

Deaths and death rates for the 10 leading causes of death in specified age groups: USA, 1999 ( Rates per 100,000) All causes2,391, Diseases of.

Classification of Bacteria. Taxonomy The science of classification.

Virulence factors of Bacteria that cause disease Submitted By : Nesren alkakhrass Supervised By: Dr. Abedelraouf A. Elmanama ( Ph.sc Microbiology.

Copyright © 2010 Pearson Education, Inc. Chapter 11 The Prokaryotes: Domains Bacteria and Archaea.

Bacterial Quorum Sensing Many species of bacteria use quorum sensing to coordinate their gene expression according to the local density of their population.

Advanced Microbial Physiology Lecture 4 Quorum Sensing.

Aynaz Taheri 1 C. Gyles and P. Boerlin. * Transfer of foreign DNA * Mechanisms of transfer of DNA * Mobile genetic elements (MGE) * MGEs in the virulence.

Introduction to the CGE servers

GRAM POSITIVE & GRAM NEGATIVE BACTERIA

BIO 411 – Medical Microbiology Chapter 9 Commensal and Pathogenic Microbial Flora.

Sequencing capacitiesacademic company based microarray facilitiesacademic company based bioinformaticsacademic proteomic facilitiesacedemic Genome Research.

Kingdom Monera Archaebacteria Methanogens Swamps, Intestines Thermophiles Hydrothermal Vents Halophiles Salt Lake, Utah Eubacteria (peptidoglycan) Autotrophs.

Prokaryotic Cell “before” Nucleus (has no nucleus) No membrane bound organelles 3.5 billion years Unicellular Circular DNA Contain a cell wall Eukaryotic.

BACTERIA UBIQUITOUS IN NATURE UNICELLULAR LIGHT MICROSCOPIC MICROMETERS IN DIAMETER; MICROMETERS IN LENGTH PROCARYOTIC IN CELL STRUCTURE.

Beta lactam antibiotics & Other cell wall synthesis inhibitors

Relationships Between Bacteria and Clinical Features of Disease

Division of Bacterial, Parasitic and Allergenic Products Jay E. Slater, MD Director, DBPAP.

ERA-NET PathoGenoMics Meeting Bonn 7-8 April, 2005 Research topics of interest in the Area of Genomics of Bacterial and Fungal Pathogens of Humans Prof.

1 GRAM POSITIVE & GRAM NEGATIVE BACTERIA Dr. Fawzia Al-O tabi.

F INDINGS National Institutes of Health National Institute of General Medical Sciences Bugging the Bugs Microbial Geneticist Bonnie Bassler: Investigating.

Integrating Genomics Throughout the Curriculum, with an Emphasis on Prokaryotes Jeffrey D. Newman Lycoming College May 20, 2002.

Commercial Production of Antibiotics

Quorum Sensing as a Potential Antimicrobial Target

Semantics of Quorum Sensing and Quorum Quenching

Escherichia coli O157:H7 and Shiga toxin-converting bacteriophage Edward G. Dudley, Ph.D Department of Food Science

Hantavirus Threat Nisus Solution. Hantavirus Hantavirus pulmonary syndrome (HPS) is a deadly disease transmitted by infected rodents through urine,

Bacterial Infection of Cardiovascular system By Dr. Humodi A. Saeed Associate Prof. of Medical Microbiology College of Medical Laboratory Science Sudan.

Host-Parasite Relationship Normal Flora Pathogens Infections Opportunistic pathogens.

Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings PowerPoint ® Lecture Slide Presentation prepared by Christine L. Case M I C R.

Division of Bacterial, Parasitic and Allergenic Products Jay E. Slater, MD Director, DBPAP.

Virulence Factors & Features Important in Disease.

Kingdom Monera Archaebacteria Methanogens Swamps, Intestines Thermophiles Hydrothermal Vents Halophiles Salt Lake, Utah Eubacteria (peptidoglycan) Autotrophs.

Bacterial Infections HB Bacteria are: Unicellular Unicellular Small (1-4  m) Small (1-4  m) Prokaryotes- no nucleus or membrane bound organelles.

The δ, ε, & γ- proteobacteria.

The V. fischeri Autoinducer N-(b-ketocaproyl)-L-homoserine lactone.

GRAM POSITIVE & GRAM NEGATIVE BACTERIA

1 GRAM POSITIVE & GRAM NEGATIVE BACTERIA Dr. Fawzia AL-Otaibi.

Mohammad Emaneini, PhD Assistant Professor in Microbiology Department of Microbiology School of Medicine Tehran University.

Biofilms. Dental plaque Problems associated to biofilms 1- Physical effects: ships, tubing… 2- Metal surface corrosion 3- Development on medical implants.

Kingdom Monera Archaebacteria Methanogens Swamps, Intestines Thermophiles Hydrothermal Vents Halophiles Salt Lake, Utah Eubacteria (peptidoglycan) Autotrophs.

Reservoirs and vectors Reservoirs Animal, soil, water etc - source of infection. Vectors Arthropods, especially fleas, ticks, and mosquitoes Mechanical.

GRAM POSITIVE & GRAM NEGATIVE BACTERIA

Biofilms Dense aggregates of surface-adherant microorganisms embedded in an exopolysaccharide matrix. 65% of human bacterial infections involve biofilms!

Do Cyanobacteria Communicate With Each Other? Bacterial Communication Quorum Sensing Important to perform group functions Secretion of toxins to kill.

Introductory medical bacteriology Chien-Ming Li MD, Ph.D.

Understanding Bacteria Bacteria Everywhere. Food Safety and the Battle with Bacteria  The United States has one of the most safest food supplies in world.

Bacteria-Host Relationships commensals opportunistic pathogens primary pathogens.

MICROBIOLOGIA GENERALE Prokaryotic genomes. The prokaryotic genome.

MICROBIOLOGY PRESENTATION BY Momen ali khan. Staphylococcus Streptococcus Enterococcus faecalis.

Small RNA Control of Quorum Sensing Part I

Microscopic (Eukaryotic cells are at least 10x bigger) Unicellular

Prof. Ramesh Wigneshweraraj

Resuscitation can be delayed using the QS inhibitor Cinnamaldehyde

MICROBIOLOGIA GENERALE

The 12 “Most Unwanted” Bacteria

INTRODUCTION Vibrio fischeri Hawaiian Bobtail Squid.

Presentation transcript:

Small Talk Cell-to-Cell Communication in Bacteria

Prokaryotic (Bacterial) Cells

V. fischeri Quorum Sensing LuxI Luciferase Genes LuxR

Quorum Sensing In Bacteria LuxI Target genes LuxR

Acyl-Homoserine Lactone Autoinducers

LuxI-LuxR Quorum Sensing Systems V. fischeri P. aeruginosa A. tumefaciens E. carotovora Bioluminescence Virulence Factors, Biofilms Mating, Transfer of Mobile DNA Virulence Factors, Antibiotic Synthesis

LuxN LuxQ AI-1 AI-2 Luciferase Genes Light Quorum Sensing in V. harveyi

LuxN AI-1 AI-2 V. harveyi Reporter Strain AI-1 Luciferase Genes Light

LuxQ AI-1 AI-2 Luciferase Genes Light V. harveyi Reporter Strain AI-2

Quorum Sensing in E. coli and Salmonella

Actinobacillus actinomycetemcomitans Actinobacillus pleuropneumoniae Actinobacillus suis Bacillus anthracis Bacillus halodurans Bacillus subtilis Borrelia burgdorferi Campylobacter jejuni Clostridium acetobutylicum Clostridium difficile Clostridium perfringens Deinococcus radiodurans Escherichia coli MG1655 Escherichia coli O157:H7 Enterococcus faecalis Haemophilus influenzae Helicobacter pylori Klebsiella pneumoniae Lactococcus lactis Leuconostoc oenos Listeria monocytogenes Mannheimia haemolytica Neisseria gonorrhoeae Neisseria meningitidis Pasteurella multocida Pasteurella trehalosi Porphyromonas gingivalis Proteus mirabilis Salmonella paratyphi Salmonella typhi Salmonella typhimurium Shewanella putrefaciens Shigella flexneri Staphylococcus aureus Staphylococcus epidermidis Streptococcus gordonii Streptococcus mutans Streptococcus pneumoniae Streptococcus pyogenes Vibrio cholerae Vibrio harveyi Vibrio parahaemolyticus Vibrio anguillarum Vibrio vulnificus Yersinia pestis Bacteria That Contain luxS (A sample of 534 genomes sequenced)

Targets of AI-2 Actinobacillus actinomycetemcomitansPeriodontal virulence, Mixed-species biofilms Bacillus anthracisGrowth Bacillus cereusBiofilms Bacillus subtilisDevelopment Borrelia burgdorferiVirulence Campylobacter jejuniMotility, Toxins Clostridium difficileVirulence, Toxins Clostridium perfringensVirulence, Toxins Escherichia coli K12AI-2 transport, Biofilms, Cell division, DNA processing, Iron uptake Escherichia coli EHEC O157:H7Virulence, Type III Secretion, Motility Escherichia coli EPECMotility, Type III Secretion Haemophilus influenzaeVirulence, Cell invasion Helicobacter pyloriMotility, Biofilms Klebsiella pneumoniaeBiofilms Lactobacillus reuteriBiofilms in mice Listeria monocytogenesBiofilms Mannheimia haemolyticaVirulence, Encapsulation, Adhesion Neisseria meningitidisVirulence, Bacteremia Photorhabdus luminescensAntibiotic production Porphyromonas gingivalisVirulence, Proteases Hemin acquisition, Stress response Salmonella typhiBiofilms Salmonella typhimuriumAI-2 transport, Biofilms Serratia marcescensVirulence in C. elegans, Hemolysin, Antibiotic production Shigella flexneriVirB (virulence factor) Staphylococcus epidermisVirulence, Biofilms Streptococcus gordoniiMixed-species biofilms Streptococcus mutansVirulence, Biofilms, Competence, Stress response Streptococcus pneumoniaeVirulence, Persistence, Competence Streptococcus pyogenesVirulence factors, Protease, Hemolysin Vibrio anguillarumProtease Vibrio choleraeVirulence, Toxins, Biofilms Vibrio fischeriBioluminescence, Colonization Vibrio harveyiBioluminescence, Biofilms, Siderophore, Virulence, Type III Secretion, Protease

LuxN LuxQ AI-1 AI-2 Quorum Sensing in V. harveyi Luciferase Genes Light

4,5-Dihydroxy-2,3-Pentanedione Inter-Species Communication The Structure of AI-2

LuxN LuxQ AI-1 AI-2 Luciferase Genes Light LuxS Making Antibiotics Based on AI-2

AI-2 Analog CHEMISTRY Making Antibiotics Based on AI-2

LuxQ AI-2 Luciferase Genes No Light AI-2 Quorum Sensing in V. harveyi

LuxN AI-1 Luciferase Genes Light AI-1 Quorum Sensing in V. harveyi

Virulent Bacteria Test Substance Inject Making Antibiotics Based on AI-2 Dead or Alive

Quorum Sensing In Bacteria Bacteria Talk to Each Other Bacteria Distinguish Self from Other Opportunities for Novel Biotechnological Applications To Impede/Enhance Quorum Sensing Controlled Functions Quorum Sensing Allows Bacteria To Be Multi-Cellular Similar to Higher Organisms Many More Molecules Remain To Be Discovered i.e., molecules that tell “who” the other is Natural Anti-Quorum Sensing Strategies Already Exist Multiple Languages i.e., intra- and inter-species communication