This meeting is possible with the support of : 9-11 October October 2008 in Saint-Etienne, FRANCE Information : Dr Claude LAMBERT, Immunology Lab; University Hospital Saint-Etienne Pole de Biologie- Pathologie; F42055 Saint-ETIENNE cedex 2; FRANCE ; tel : ; fax : Registration before 8th september 2008 Standardisation and validation of cellular in vitro testing as support for the diagnosis of allergy Allergy may be difficult to diagnose and skin tests are not always sufficient for confirmation. In vitro cellular tests have been shown as an efficient alternative for several years. During the last decade the flow cytometric Basophil Activation Test (BAT) has been developed as a new, quantitative more precise method. The test has been well documented and few ready to use tools have been developed. The lymphocyte activation test for drug allergy will also be discussed. Inter laboratory and intersystem reproducibility of the tests needs to be established, and they have to be validated for clinical utility. This meeting aims to bring together academic biologists, clinicians and industrial scientists involved in flow cytometry in allergy to mature these tests for use in routine healthcare. Scientific committee Colette CHAPPARD Charles DZVIGA Didier EBO Claude LAMBERT Organizing committee Charles DZVIGA Véronique CASU Daniel LOUPIAC Claude LAMBERT

St Etienne, 9-11 October 2008 Friday 10 october 2008 Progress of each group 8h45- 10h30 Plans, composition and preliminary results Technical issues ………………………….HJ Hoffmann (DK) Hymenoptera venom ….……D Ebo (BE) Food ……………………………...……….E Knol (NL) Drug induced hypersensitivity Betalactams ………………………...L Mayorga (ESP) Myorelaxant ………………………...J Saint-Laudy (FR) Non steroid anti-inflammatory drugs …M Sanz (ESP) Auto-immune urticaria ………………M Ferrer (ESP) (10 min + 5 min discussion) Coffee Break 11h00- 12h30 : METHODS AND STRATEGIES (10 min + 5 min discussion). Lunch Break BIOLOGICAL PERSPETIVES 14h00- 15h30 BIOLOGICAL PERSPETIVES Review on basophiles …………………….. E Knoll (NL) News on basophiles analyses ……………. D Ebo (BE) Coffee Break CLINICAL PERSPETIVES 16h00- 18h00 : CLINICAL PERSPETIVES Venom allergy and desensitization protocols J Birnbaum (FR) Drug allergy ………………………………… to be announced Auto-Immune urticaria ……………………. to be announced 19h00 Dinner at the Musée le l’art moderne Saturday 11 October 2008 ANALYTICAL ISSUES 8h30- 10h00 WORKSHOPS ON STRATEGY Technical Issues, Clinical protocols Coffee Break 10h30- 12h00 WORKSHOPS ON SPECIFIC ALLERGIES Venom; Food; Drugs; Autoimmunity Lunch Break 14h00- 16h00 Round table methodological issues HJ Hoffmann (Denmark), D Ebo, (Belgium), E Knol, L Mayorga, (Spain), J Saint-Laudy, (France), M Sanz, (Spain), M Ferrer, (Spain) Coffee Break 16h30- 17h30 Conclusion & agenda for the next meeting Tasks, milestones, interaction with EAACI clinical interest groups Oral communications will be selected on abstract to present results during sessions or workshop

St Etienne, 9-11 October 2008 Registration Before 8th September after Before 8th September after Participant : 125 € 175 € The registration fee covers participation in the workshop; lunches and coffee breaks; gala dinner at the museum and documents SURNAME: ………………………………………………………… FIRST NAME: …………………………………………..…………. ADDRESS: …………………………………………….…………… ………………………………………………………………………… ….…………………………………………….…………………..…… POSTCODE: ……………… TOWN: ……….………………. COUNTRY: …………. ………………………………….. FAX. : ………………………………………. TELEPHONE: …….………………………………….. ACCOMPANIED BY: ………………………………… Arrival Departure date ….. / 10/2008 ….. / 10/2008 Time …. h …. ….. h …… St Etienne TGV station ………..  Saint-Exypery Lyon airport …….  Car ……………………………..  Information: Dr Claude LAMBERT tel : ; fax : ;  Bank check order to ALEPOC :  VISA or  MASTERCARD n° ………………………………….. expiry date: ……/…….. + last 3 digits at the back of your card If your credit card details are incomplete, we will not process your registration. How to get to Saint Etienne ? - Fly to Lyon Saint-Exupery and take a shuttle either to Lyon part-Dieu train station and train to Saint Etienne, or a direct shuttle to St Etienne - Take theTVG from Paris (Gare de Lyon or Roissy Airport) to St. Etienne (3 hours) If we get your arrival schedule, we can arrange for transfers. Hotel booking : use the attached form The meeting will take place at “le Grand Cerlce” in the centre of Saint-Etienne