Mrs. K. Johnson LCHS

Fingerprinting Activity Listen to the directions. You will work in lab groups. Materials: 1 ink pad 1 piece of paper Analyze results, examine differences.

What is Genetic Engineering? (Write this down.) Genetic engineering is the transfer of genes from one organism to another. We can recombine pieces of DNA from different organisms. WHY? To cure diseases (still in progress) To treat diseases Food Crops Livestock Basic Research Tool

Gene Splicing (Write this down.) Restriction enzymes cut DNA at precise spots. The restriction enzyme used most often is Endonuclease. It cuts the desirable DNA chunk out so it can then be put into another organism.

The jellyfish glow gene has become a powerful tool for studying molecules inside living cells.

Gene Splicing Example: The Glowing Tobacco Plant R.E. cuts the “glowing” gene from a firefly’s DNA. R.E. cuts open the tobacco plant’s DNA and the “glowing” gene is placed inside. Ligase enzyme is used to seal or glue the DNA back together. End result: Recombinant DNA

52 % of cotton grown last year was genetically engineered with a bacteria gene to resist bugs.

Glow in the Dark Mouse A glowing mouse that had the glowing jellyfish gene implanted.

A cat in Korea was engineered to glow in the dark. How could this help with disease research? The cat’s skin glows.

DNA Sequencing (Write this down!) Use a single strand of DNA Cut it into sequences using a restriction enzyme Gel electrophoresis p. 356 Fig. 10

A Genetically Engineered Tomato, so what’s the BIG DEAL?

Just as you each had different fingerprints, you also have different DNA sequences, DNA fingerprints. You will use those differences in the following activity.

Online DNA Fingerprinting Go to this website: nova/sheppard/analyze.html Follow the instructions!

Turn in one half-sheet for your lab group Answer: Who is the… Holographic Lollipop Licker?