Real-time PCR automated solution for food pathogen testing Jean-Philippe Tourniaire – International Product Manager Lab Innovations, Birmingham, November 6th 2013

iQ-Check Prep Solution goals Straightened our global offer on iQ-Check Food pathogen testing Provide technically advanced solutions to medium to high throughput laboratories Provide confidence and peace of mind with a cost effective solution

iQ-Check Prep Solution Concept Complete automation of the iQ-Check Easy protocols From the enriched sample to the PCR plate ready Two plates handled at the same time Run of up to 4 different parameters on a plate Compatible with all the iQ-Check Food kits Optimal integrated solution with user-friendly interface: CFX Manager software IDE Partnership with Hamilton http://www.hamiltonrobotics.com, a WW leader in automation

Automation Concept Automated

The iQ-Check Prep System and his Deck

Deck description Rack 1 mobile Rack 2 Non mobile Rack 3 mobile Rack 4 1000 µL tips Agitator / incubator DW plate 2 for extraction 50 µL tips Gripper DW plate 2 for samples Tubes for PCR mix 1 2 3 4 5 6 7 8 Waste DW plate for dilutions (Transfer) PCR plate loading place Lysis bottles 2 3 DW plate 1 for extraction 1 4 PCR plates 1 & 2 DW plate 1 for samples 1 2 3 4 1000 µL tips PCR reagents kits Water container

Technology 4 channels Air displacement pipetting principle Tips pick-up with CO-RE Technology Type of tip recognition

Technology Liquid Level Detection: LDD Monitored Air Displacement: MAD Added value of the conductive tips: Liquid level sensing (aspiration / dispensing) for increased safety and confidence Monitored Air Displacement: MAD

iQ-Check Prep User Interface Two computers and software needed: CFX ManagerTM Software IDE v2.0 : handles the CFX96 Deep WellTM Touch (and MiniOpticonTM) and allows the plate set-up for the iQ-Check Prep

CFX Manager Software IDE v2.0

iQ-Check Prep Software - User Interface iQ-Check Prep Software v1.3: gets information from the CFX Manager IDE v2.0 and handles the protocols on the iQ-Check Prep instrument. The user is guided through the different steps to start the run : refill of consumables and reagents

Throughput Capacities / Workflow Highest throughput configuration: 2 x 94 samples / prep run : about 2 x 70 min 2 hrs PCR Process 6 plates in 8 hrs Time duration of different protocols or steps Complete run : 1h10 min DNA already extracted: 30 min 1/10 dilution: + 15 min Lysis reagent dispensing: 8 min PCR mix preparation and dispensing: 19 min Cooling the Deep Well plate (DNA extract): 25 min

Throughput Capacities / Workflow Configuration 1: 1 iQ-Check Prep + 1 CFX96 Deep Well Touch IDE More than 250 samples tested in 8 hrs Configuration 2: 1 iQ-Check Prep + 2 CFX96 Deep Well Touch IDE More than 500 samples tested in 8 hrs

Absence of Cross Contamination One of the major concern for this project: tested many times with highly positive and negative samples Perfectly clean results and deck!!!

AFNOR and AOAC-RI Validations

Features and Benefits Benefit Feature Complete transparency and possibility to handle manually the samples if needed Manual protocol automatized without modification Recognized solution with validated performances AFNOR and AOAC-RI validated solutions High confidence and traceability Handles complete DNA extraction and PCR plate preparation Improved confidence in liquid handling Conductive tips, pipetting control with liquid level sensing

Features and Benefits Benefit Feature Avoid cross contamination risks with aerosols and droplets CORE technology Limited maintenance and wastes: no tubing, pump, liquids,…. Air displacement technology Faster than any other automation system, ideal for high throughput labs Process 96 tests in a little more than 3 hours (more than 250 or 500 samples / 8 hours) Easy to learn and to use Optimized traceability between the iQ-Check Prep and the CFX96 Touch Deep Well real-time PCR instrument Handled through the use of the CFX Manager IDE and the iQ-Check Prep software

Features and Benefits Benefit Feature Optimized flexibility to handle all major parameters Compatible with all iQ-Check Food kits and up to 4 parameters / plate Save time : no need to dissolve, rehydrate, defreeze,…. Ready to use reagents No disruption of lab workflow: reallocate your lab resources Proceed with samples by batch Save time in handling and managing your results LIMS connection, email results notification Save time for support and anticipate any maintenance action Remote control to monitor log files for preventive maintenance and support

Key Benefits Easy to use LIMS connection to import & export data Improved throughput capacities Improved traceability Increased accuracy: less handling errors Save technician handling time Simple transposition of the manual protocol (not a black box) Pipetting control Limited maintenance (air displacement) AFNOR and AOAC validated

Feedback From Users J. P., evaluator “we really like the Prep station …. The advantages are that it virtually eliminates the potential for liquid handling and cross contamination errors. The operation of the Prep station is straightforward and the software is easily navigable providing the operator with prompts, diagrams and help/troubleshooting screens. The PCR detection software is also user-friendly and intuitive….” C.S., evaluator Easy to use Great and improved PCR results Save minimum 1 hr technician time / day From competitor “It’s hard to make it simple, and you did it!”

iQ-Check Prep Solution Thanks a lot for your attention!