1. Logical In-Vial Dilution with Ultrafiltration for Anion Analysis IC Application Work AW US6-0149-012011 Presented by Carlos Bazán 1

2. Overview Dosino technology provides a highly efficient and accurate method for performing dilutions in-line; saving customers time, money, and reducing human error Previous configurations required external mixing with added components and added $$ The In-Vial Dilution configuration can perform dilutions in an empty sample vial and utilizes the Dosino to mix the diluted sample Intelligent re-dilution of samples above the calibration range is possible Next-Vial Dilution allows the next sample in the series to be diluted while the current sample is still running, maintaining high sample through-put 2

3. Components needed 881, 882, or 850 IC 858 Sample Processor with peristaltic pump (2.858.0020) 800 Dosino with 10 mL Dosing Unit IC Equipment for Ultrafiltration (6.5330.010) T-Connector 3 X UNF 10-32 (6.2744.290; diverts flow for dilution or filtration) In-line check valve (6.2744.190; prevents Dosino from pulling from ultrafiltration cell waste) 10 mL Transfer Capillary (6.1562.130) Wash station for IC (6.2841.100) 3

4. Basic function Sample is aspirated into the transfer capillary by the Dosino. The in-line check valve prevents UF cell waste from being aspirated. 4

5. Basic function The aspirated sample and a quantity of UHP H 2 O to complete the dilution are dispensed into an empty sample vial. 5

6. Basic function The diluted sample is aspirated and dosed several times to mix it completely. 6

7. Basic function The peristaltic pump moves the diluted sample through the ultrafiltration cell and to the IC. 7

8. MagIC Net Sample Table Injections= 1 for normal analysis, 2 when an intelligent re-dilution check should be performed Dilution= the Manual Dilution Factor for hand-dilutions made Value 1= the Dosino Dilution Factor; the dilution the instrument will perform Value 2= the Dilution Vial; vial position the dilution is performed in Value 3= the Intelligent Dilution Vial; vial position for intelligent re-dilution when needed 8

9. Features: Calibration Calibration from one stock standard; levels made by variable dilution Makes standard prep easier, saves time, reduces human error Dilution Factor (Value 1) 200100502010 9

10. Features: Next-Vial Dilution Prepares the dilution of the next sample in the series while the previous sample is still running Reduces the overall dilution time needed for a sample series; very valuable for labs needing high through-put Can be used with intelligent re-dilution; during the re-dilution run the next sample is prepared Uses the SD.NEXT values in the program to access the POSITION, VALUE1 and VALUE2 of the next line in the sample table 10

11. Features: Multi-component Intelligent Re-dilution Software can evaluate which out of the entire list of component ions is most above the calibration range Re-dilutes samples accordingly 11 If this is not an intelligent dilution run (Injection=1),or the Area of Fluoride =0,then Autodilution Factor for Fluoride=0. If it is an intelligent dilution run (Injection=2) then Autodilution Factor for Fluoride = (Concentration of Fluoride in current run / (Concentration of Fluoride in the highest standard X the Manual Dilution Factor )) X the Dosino Dilution Factor

12. Features: Multi-component Intelligent Re-dilution 12

13. Performance: Calibration Linearity Low calibration RSD Linear across a broad calibration range 13

14. Performance: Dilution Accuracy 99%+ Accuracy compared to a 1:10 hand-dilution 96%+ Accuracy compared to a 1:100 hand-dilution (1:100 is maximum advisable Dosino-prepared dilution with 10 mL Dosing Unit) 14

15. Performance: Reproducibility Approximately 1.5% RSD on 6 replicate injections of 1:100 dilution (with lower RSD at lesser dilution factors). 15

16. Performance: Intelligent Re-dilution System can re-dilute samples based on which ion is most above the calibration range; can compare all analytes in component table Highest standard Samples FluorideClN0 2 as NBrNO 2 as NP0 4 as PSO 4 Std 53.972 ppm100.463 ppm1.507 ppm1.977 ppm10.072 ppm9.699 ppm98.899 ppm 16

17. Market Potential A large percentage of environmental and QC laboratories must routinely perform sample dilutions User proficiency is going down, so reliable in-line, automated methods for dilution and standard preparation are highly valuable Automatic re-dilution of samples above the calibration range saves time, especially on samples with 48 hr. hold-time L OW COST OF THE SYSTEM MEANS WE SHOULD SELL A LOT OF THESE TO A LOT OF USERS ! 17

18. Acknowledgements Jay Kim for invaluable contributions to programming of the intelligent dilution and next-vial dilution components of the method Dr. Shibu Paul for the initial development request and continuing drive to perfect the method Dr. Hari Narayanan for advice and guidance Larry Tucker for suggestions on sample flow path plumbing and wisdom on Dosino capabilities Dr. Stuart Procter for method testing during technical training sessions 18

19. Questions? 19