Experiment 4- Gene Expression Study in Arabidopsis Thaliana.

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Presentation transcript:

Experiment 4- Gene Expression Study in Arabidopsis Thaliana

RNA Blot Quantitative RT-PCR Microarrays Tools For Studying Differential Gene Activity

What Are The Major Differences Between DNA and RNA?

RNA Has a Uracil Instead of Thymine

RNA Has Ribose Sugar in Nucleotide

Eukaryotic RNAs Are Single-Stranded Polynucleotides Nucleotides Joined By Phosphodiester Bonds Like All Nucleic Acids Order 5’  3’ Leads to Function: Co-Linear With Gene Sequence

RNA Has Intra-Strand Double Helices or Secondary Structure

Total RNA Isolation Workflow Collecting plant materials (1-6) Lysis (7-11) (12-16) Washing (21-32) Precipitation & Binding (17-20) Elute RNA (33-40) Determining Concentration 1. RNA Isolation 2. Remove Genomic DNA RNase-Free DNase I 3. Electrophoresis Determining Quality of Isolated RNA

Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System

Determining Quality of Isolated Total RNA Using A Capillary Gel Electrophoresis System- Part II Leaf Silique

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