E-300 fully automated ELISA preparation and analysis workstation

Overview The E300 is a rugged, industrial quality, fully automated workstation for ELISA preparation and analysis. It is a high capacity unit allowing up to 320 samples, 6 plates plus hotel storage for continuous feed and longer walk-away time. It incorporates many features to maintain test integrity and custody of sample. These include automated barcoding of samples, reagents and controls, clot detection, rapid measurement of actual volume in pipette tip – before and after pipetting and automated sample drawer. The 4 pipette probes and separate plate handling arm make it the fastest unit available. The unit includes an embedded computer and touchscreen mounted on workstation making an ideal interface for our simple, intuitive software. Features Rugged fully enclosed unit Dual Arm Workstation for high test throughput 4 Pipette Probes with integral air-displacement pumps Long reach Plate/ Tipbox handler for full Hotel access Disposable tips for zero carryover or optional fixed tips Hotel stores 9 tipboxes and up to 18 additional plates – conveyors allow easy introduction of supplies Hotel storage allows continuous feed and longer walk-away time Automated barcoding of samples, reagents, controls and plates Automated sample rack gives custody control to workstation Ratio of samples to reagents and controls can be varied – custom racks available Typical setup samples, 48 controls, 36 reagents (or 9 troughs) Level and Clot detection Pipetting Verification – measures actual volume in pipette tip Up to 3 Incubator/ Vortexing modules – actual orbital vortexing Plate Washer up front for easy access and observation Integrated Plate Reader External dual cylinder high capacity vacuum pump Buffers on drawer for easy access – weight sensor for onscreen display of volume Embedded computer and Touchscreen monitor Intuitive, graphical software also allows custom assays

Sample Tray extends automatically for easy loading and custody of sample. Up to 324 samples,64 reagents and 64 controls share the same rack so ratios can be varied. All racks are automatically barcoded. Custom racks are available.

Automated barcoding of samples, reagents and controls. Barcode arm separates trays to barcode in between rows. Hard to read barcodes retry on user selected criteria.

Wash Station located up front for simplified adjustment and observation. Wash head is coaxial type for complete evacuation of wells. Sweep can be circular or 1- 8 point. Vacuum provided by high capacity 2 stage piston vacuum pump.

Extended reach plate handler automatically replaces tipboxes and plates (if desired) from Hotel Storage. Up to 9 tipboxes and 18 plates can be stored and replaced on a continuous basis from right exterior of unit. The plate handler also transfers plates from Preparation Area to Wash Station, Plate Reader, Heated Incubator/ Vortexor and Covered Room Temperature Incubation Stations

Pipettors shown entering Pipetting Verifier. Actual measurement of volume before and after pipetting rapidly provides absolute confirmation that sample was aspirated from sample tubes and dispensed into test plate. User can select whether to check aspiration, dispense or both for samples and /or reagents and controls.

Independently spaced, 4 pipettor unit allows rapid processing of samples. Samples can be accessed in any order. All pipettors can take either sample or reagent tips. Plate Rotator (shown in forefront) allows portrait or landscape pipetting in preparation station.

Workstation can be equipped with up to 3 Incubator/ Vortexors. Each can hold 2 plates if needed. These are true orbital vortexors with user programmed speed, duration and cycles. Full temperature range of ambient plus 4 °C up to 50°C +/- 1%.

Replacement of Tipboxes and Plates (if desired) is done from right exterior of workstation without the need to pause assay. Conveyors on each row assist in introduction of disposables and detect when rows are empty. Old boxes are put back into empty rows and user notified when supplies are exhausted. Up to 9 additional tipboxes can be stored (plus 3 on the deck) and 9 plates plus 9 deepwells (or 18 plates).

Buffer bottles are accessed with convenient slide out drawer. Bottles are standard Nalgene Labware and are sitting on weight sensors with levels displayed on software screen. Levels can also be seen through translucent plastic.

Intuitive, easy to use software with a touchscreen interface makes operation of the E-300 simple and straightforward. Different skill levels accommodate different user requirements. You can choose a wizard, prompts every input or for more advanced users select several operations on a more condensed screen. Writing new assays can also be targeted specifically for the most common assays or a broader selection of options is available for special assays.

Specifications Maximum 320 Samples Maximum 60 Reagents/ Diluents Maximum 80 Controls 4 Buffer containers – 2 liter DI Wash Water – 4 liter Waste Container – 12 liter 6 plates on deck – up to 18 additional in Hotel 1 reagent tipbox and 2 sample tipboxes on deck – 9 additional in Hotel 2 arms – 4 pipettors on Arm 1 and Plate Handler on Arm 2 Throughput – 540 tests in 4 hour period

About US FMP Products was formed in 2005 by the principles in Cyberlab, Inc (purchased by Gilson, Inc in 2000). We have over 20 years experience in manufacture and design of laboratory automation products and accessories. We have done a wide range of customer specified engineering solutions and applications. All equipment is designed, manufactured and assembled in the USA. Contact Information: Sales/ Marketing FMP Products Tom Friedlander Engineering Walter Meltzer