1. Real-time PCR automated solution for food pathogen testing
Jean-Philippe Tourniaire – International Product Manager
Lab Innovations, Birmingham, November 6th 2013

3. iQ-Check Prep Solution goals
Straightened our global offer on iQ-Check Food pathogen testing
Provide technically advanced solutions to medium to high throughput laboratories
Provide confidence and peace of mind with a cost effective solution

4. iQ-Check Prep Solution Concept
Complete automation of the iQ-Check Easy protocols
From the enriched sample to the PCR plate ready
Two plates handled at the same time
Run of up to 4 different parameters on a plate
Compatible with all the iQ-Check Food kits
Optimal integrated solution with user-friendly interface: CFX Manager software IDE
Partnership with Hamilton a WW leader in automation

5. Automation Concept
Automated

6. The iQ-Check Prep System and his Deck

7. Deck description Rack 1 mobile Rack 2 Non mobile Rack 3 mobile Rack 4
1000 µL tips
Agitator / incubator
DW plate 2 for extraction
50 µL tips
Gripper
DW plate 2 for samples
Tubes for PCR mix
Waste
DW plate for dilutions (Transfer)
PCR plate loading place
Lysis bottles
DW plate 1 for extraction
PCR plates
1 & 2
DW plate 1 for samples
1000 µL tips
PCR reagents kits
Water container

8. Technology 4 channels Air displacement pipetting principle
Tips pick-up with CO-RE Technology
Type of tip recognition

9. Technology Liquid Level Detection: LDD Monitored Air Displacement: MAD
Added value of the conductive tips:
Liquid level sensing (aspiration / dispensing) for increased safety and confidence
Monitored Air Displacement: MAD

10. iQ-Check Prep User Interface
Two computers and software needed:
CFX ManagerTM Software IDE v2.0 : handles the CFX96 Deep WellTM Touch (and MiniOpticonTM) and allows the plate set-up for the iQ-Check Prep

11. CFX Manager Software IDE v2.0

12. iQ-Check Prep Software - User Interface
iQ-Check Prep Software v1.3: gets information from the CFX Manager IDE v2.0 and handles the protocols on the iQ-Check Prep instrument. The user is guided through the different steps to start the run : refill of consumables and reagents

13. Throughput Capacities / Workflow
Highest throughput configuration:
2 x 94 samples / prep run : about 2 x 70 min
2 hrs PCR
Process 6 plates in 8 hrs
Time duration of different protocols or steps
Complete run : 1h10 min
DNA already extracted: min
1/10 dilution: min
Lysis reagent dispensing: 8 min
PCR mix preparation and dispensing: 19 min
Cooling the Deep Well plate (DNA extract): 25 min

14. Throughput Capacities / Workflow
Configuration 1:
1 iQ-Check Prep + 1 CFX96 Deep Well Touch IDE
More than 250 samples tested in 8 hrs
Configuration 2:
1 iQ-Check Prep + 2 CFX96 Deep Well Touch IDE
More than 500 samples tested in 8 hrs

15. Absence of Cross Contamination
One of the major concern for this project: tested many times with highly positive and negative samples
Perfectly clean results and deck!!!

16. AFNOR and AOAC-RI Validations

17. Features and Benefits Benefit Feature
Complete transparency and possibility to handle manually the samples if needed
Manual protocol automatized without modification
Recognized solution with validated performances
AFNOR and AOAC-RI validated solutions
High confidence and traceability
Handles complete DNA extraction and PCR plate preparation
Improved confidence in liquid handling
Conductive tips, pipetting control with liquid level sensing

18. Features and Benefits Benefit Feature
Avoid cross contamination risks with aerosols and droplets
CORE technology
Limited maintenance and wastes: no tubing, pump, liquids,….
Air displacement technology
Faster than any other automation system, ideal for high throughput labs
Process 96 tests in a little more than 3 hours (more than 250 or 500 samples / 8 hours)
Easy to learn and to use
Optimized traceability between the iQ-Check Prep and the CFX96 Touch Deep Well real-time PCR instrument
Handled through the use of the CFX Manager IDE and the iQ-Check Prep software

19. Features and Benefits Benefit Feature
Optimized flexibility to handle all major parameters
Compatible with all iQ-Check Food kits and up to 4 parameters / plate
Save time : no need to dissolve, rehydrate, defreeze,….
Ready to use reagents
No disruption of lab workflow: reallocate your lab resources
Proceed with samples by batch
Save time in handling and managing your results
LIMS connection, results notification
Save time for support and anticipate any maintenance action
Remote control to monitor log files for preventive maintenance and support

20. Key Benefits Easy to use LIMS connection to import & export data
Improved throughput capacities
Improved traceability
Increased accuracy: less handling errors
Save technician handling time
Simple transposition of the manual protocol (not a black box)
Pipetting control
Limited maintenance (air displacement)
AFNOR and AOAC validated

21. Feedback From Users J. P., evaluator
“we really like the Prep station …. The advantages are that it virtually eliminates the potential for liquid handling and cross contamination errors. The operation of the Prep station is straightforward and the software is easily navigable providing the operator with prompts, diagrams and help/troubleshooting screens. The PCR detection software is also user-friendly and intuitive….”
C.S., evaluator
Easy to use
Great and improved PCR results
Save minimum 1 hr technician time / day
From competitor
“It’s hard to make it simple, and you did it!”

22. iQ-Check Prep Solution
Thanks a lot for your attention!