1. Rev 4/28/06

2. SPIFE 3000 11 analytes, with multiple gel sizes for every workload Typical throughput – up to 14 SPE and 12 IFE gels in 8 hours Used in hundreds of medical centers and reference laboratories worldwide Semi-automated electrophoresis system

3. Automated sample application system Automated reagent application for enzymatic assays SPIFE 3000 Temperature-controlled electrophoresis, incubation and drying

4. Automated staining and drying of visible analytes SPIFE 3000 Positive ID of sample tray and gel

5. SPIFE Assays Cholesterol Profile Serum Proteins Split-Beta SPE Urine Proteins Immunofixation Urine Immunofixation Hi Resolution Proteins Alkaline Hemoglobins Acid Hemoglobins CK & LD Isoenzymes Alk Phosphatase Isoenzymes CSF IgG IEF Lipoproteins

6. SPIFE IFE Built-in QC wells Up to 210 profiles in 8 hours Antisera affinity & avidity balanced for highest sensitivity with least background 1, 2, 3, 6, 9 and 15 profile gels to handle a wide range of needs Acid violet stain enhances visualization

7. SPIFE IFE Procedure Dilute automatically using the Auto Sample Handler or manually Load patients samples and applicator blades Set gel and electrodes in place in electrophoresis chamber

8. SPIFE IFE Procedure SPIFE applies samples, allows for absorption, and begins electrophoresis Blot surface of the gel and begin electrophoresis After electrophoresis, remove buffer ridges from the gel

9. SPIFE IFE Procedure Set Rigid Antisera Template in place Fast and efficient antisera application Pipette color-coded antisera for an entire profile at once

10. SPIFE IFE Procedure Absorb excess antisera from channels, preventing cross-contamination Blot gel surface, using antisera template as press and allow SPIFE to incubate and dry the gel

11. SPIFE IFE Procedure Move gel to SPIFE’s staining unit for automatic wash, stain, destain and dry Finished! All SPIFE IFE gels include integral control wells for optional use of ImmunoFix Controls to verify antisera reactivity

12. 108 slides Basics of protein structure Disease states associated with protein abnormalities Suggested protocol for evaluating monoclonals When to run, how to run, and what to look for Patterns to interpret from simple to complex Covers serum and urine New Educational CD Protein Electrophoresis and IFE An Interpretive Guide

13. Auto Sample Handler Provides positive sample ID from tube to tray to gel Primary tube sampling with liquid level sensing Bar code reader with LIS interface

14. Auto Sample Handler Automatically performs dilutions for IFE Prepares sample trays, ready for SPIFE Interfaces to QuickScan 2000 for workload listing

15. QuickScan 2000 Provide IFE and SPE results on a single report Patterns can be viewed and edited over the LAN 10,000 gel archive with easy retrieval

16. Scan anything large or small in seconds: 10-sample to 100-sample gels and L/S plates QuickScan 2000 Customized interpretive analysis and decision limits for fast reporting Simple to use – no slits or wavelengths to select

17. Multi-pattern display color- coded for in/out of range and reviewed results Build your own decision limits and interpretive comments library Comments automatically added for “normals” and coded for quick retrieval QuickScan 2000

18. Choose quantitation of split beta SPE as one or two fractions Report formats can be totally customized Profile up to 100 scans QuickScan 2000

19. SPIFE System Contact your local Helena technical representative for more information or to arrange an in-lab demonstration of the SPIFE System. 1-800-231-5663