Introducing the EASY-nLC™ Split-less, nano-flow HPLC system, for proteomics from

Copyright © Proxeon Biosystems A/S Overview The EASY-nLC™ is: Complete Configured Compact EASY to install learn operate Performance Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Complete 1 cooled autosampler 96 or 384 well plates Single or double height Wash & Waste bottles switching valves nano-flow sensor nano-litre pumps Built-in PC & software Copyright © Proxeon Biosystems A/S

EASY2-nLC™ (with flow control) 2-column AFC™ Advanced Flow Control system Solvent A Solvent B Waste Copyright © Proxeon Biosystems A/S

EASY2-nLC™ (with flow control) 1-column AFC™ Advanced Flow Control system Solvent A Solvent B Copyright © Proxeon Biosystems A/S

EASY2-nLC™ (with flow control) 2-column 1-column Waste Waste Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Compact Small footprint 35cm wide (14”) 38cm deep (15”) 45cm high (18”) Touch-screen controlled built-in computer no keyboard or mouse required Light 35 kg, all inclusive Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Easy to install Only a few cables required contact closure [network is optional] Power Software is integrated, pre-installed and up-to-date Solvent purging, air removal and column flushing is fully automated Less than 4 hours from power up to first analytical data Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Easy to learn Logical software structure Practice oriented work flow Results oriented parameters E.g. volume & flow rates, not hardware control Intuitive graphical user interface Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Easy to operate (1) What You See Is What You Get Simple design Logical layout Clear separation in compartments Modular components Easy access to all parts of instrument Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Easy to operate (2) Automated maintenance scripts Intelligent trouble shooting software Web interface for software upgrades Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Easy to operate (3) Split-less, nano-flow means: Less solvent use Less waste Robust design & Intelligent software control means: Less support Less maintenance More uptime M T W F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 M T W F S 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Example (1) Mass spectrometer Finnigan LCQ Classic Pre-column 2cm, 75µm ID, Reprosil-Pur C18-AQ. 5µm Analytical column 10cm, 75µm ID, Reprosil-Pur C18-AQ. 3µm Solvents A: 0.005% HFBA, 0.4% acetic acid, water B: 0.005% HFBA, 0.4% acetic acid, 90% acetonitril, water Amount injected 100 fmol tryptic digested BSA HPLC gradient 300 nl/min, 10-100 %B in 25 minutes 6 out of 36 consecutive runs . Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Example (2) Carry-over < 0.003% Sample pickup From 0.2µl to full loop in 0.01µl steps Loop size adjustable RSD < 0.5% Peak widths (FWHM) < 10-15s at 50% Reproducibility RT RSD < 0.7% Copyright © Proxeon Biosystems A/S

Copyright © Proxeon Biosystems A/S Summary The EASY-nLC™ is: A fully integrated and complete instrument With true nano-flow and on-line monitoring Designed from the ground up to be easy and intuitive to operate A nano-LC system for the 21st century Copyright © Proxeon Biosystems A/S