Laboratory Filtration Cassidy Markee Director of Laboratory Division

Spectrum’s Core Technology: Membrane Separation Spectrum offers products for two kinds of Membrane Separation (membrane, systems, components and accessories): 1.Membrane Dialysis – passive diffusion through a semi- permeable membrane – 100 to 1,000,000 Daltons 2.Membrane Filtration – applied pressure across a selective membrane. 3 types based on size range: a.Macrofiltration – 5 μ m and up (Woven Mesh Screens) a.Microfiltration – 0.05 – 5 μ m (Direct Flow and Tangential Flow) b.Ultrafiltration (Molecular filtration) – 5 kD to 0.05 μ m (Tangential Flow only)

Membrane Microfiltration in the Lab Two membrane configurations for Direct Flow Filtration (DFF): 1.Flat sheet DFF Devices - disadvantages 1.Less surface area - lower flux rate 2.Quicker to plug or foul 3.Lower particle load 2.Loped Hollow Fiber DFF Devices - advantages 1.Higher surface area - higher flux rate 2. Longer filter life – less fouling 3.High particle load

Mode of Action: Flatsheet DFF

Mode of Action: Hollow Fiber DFF

Spectrum’s Hollow Fiber DFF Devices Features & Benefits: Sterilizing grade 0.2 um Mixed Cellulose Ester (ME) - DynaFibre ® Looped hollow fiber membrane provides large surface area Higher flux rates and increase filtration volume Filtration from outside fiber into lumen reduces fouling and increases particle load Compact and narrow filter housing PP air vent fiber loops prevents “air lock”

Hollow Fiber Microfiltration: 4 Types of Filters MediaKap ® & MediaKap ® Plus Cold sterilization of culture medium MiniKap ® Point-of-use water filtration CultureGard ® Sterile barrier for feeding/perfusion of bioreactors DynaGard ® Syringe tip filtration

DynaGard® Syringe Tip Filters Aqueous solution filters (blue) –0.2 μm Cellulose Ester Membrane –3 sizes for 1-20 ml Organic solution filters (white) –0.2 & 0.45 μm Polypropylene Membrane –2 sizes for 1-20 ml Low fouling Irradiated or non-irradiated Narrow housing design Luer-lok™ connections

MediaKap ® & MediaKap ® Plus HF Filters MediaKap for serum-free media MediaKap Plus for serum-enriched media –Skinned DynaFibre for higher viscosity –2x filtration capacity for serum-free media 0.2 um sterilizing grade cellulose ester membrane 5 sizes – 2, 5, 10, 25 & 50 liters 0.2L – 100L (400ml/min – 2L/min) Low extractable Optional filling bell– protects sterility PP vent fiber prevents air lock

CultureGard® HF Perfusion Filters Sterile protection during culture perfusion, feeding or sampling Large surface area Minimal hold-up volume PP vent fiber prevents air lock Process highly viscous solutions Use two in series to increase duration of feeding

MiniKap® Water and Gas Filters Point-of-Use aqueous final filter Removes particles in air/gas lines as low as 0.01 μm Validated for use in clean rooms Industries served: –Electronics/Semiconductors –Medical Device Manufacturing –Biotech/BioPharm –Petrochemical

Macrofiltration: Spectra/Mesh Woven Filters 5 Material Types: Nylon Polypropylene Polyester PEEK Stainless Steel Applications: Cell & Tissue Industrial Environmental Air Particulates Pharmaceutical Research Food & Beverage Waste Mesh Opening: 5 – 1000 micron Sizes: 25, 47, 55, 90 & 150mm discs; 30x30 cm sheets

Macrofiltration Microfiltration Ultrafiltration Dialysis Reference size range and scale

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