1. ZenPure 1 Microfiltration: 1. Maximizing protein recovery. 2. Complete asepsis: eliminating hose barbs and triclamps. 3. New optimal design filter for bulk throughput

2. ZenPure 2 Zeroing in on Protein Recovery The economic impact of maximizing recovery of very high value therapeutic products from single use systems

3. ZenPure Confidential Outline The Problem: Liquid Holdup in Single Use Systems Currently Available Solutions ZenPure’s ZenCap® Zero Case Studies of Economic Impact 3

4. ZenPure Confidential The Problem: Liquid Holdup Liquid Holdup can account for significant product loss in biopharmaceutical processes. Process equipment can be designed to reduce holdup, and use of a purge-gas blow-down can remove residual holdup in many components. 4 Even lab-scale experiments can result in significant liquid holdup if no measures are taken.

5. ZenPure Confidential The Problem: Holdup in Filter Capsules Void Spaces in the filter capsule Downstream inaccessible due to filter membrane 5

6. ZenPure Confidential The Problem: High Bubble Points Microporous membrane filters resist the flow of gas through their pores once wet-out with liquid. 6 Sterilizing-Grade Membrane filters have Bubble Points in the range of 50 psi. Many pre-filters have bubble points higher than 20 psi. Air purge at these pressures is often not feasible in single-use systems. (where K is a constant based on properties of the membrane and wetting fluid)

7. ZenPure Confidential Currently Available ‘Solutions’ Ignore the problem – In many applications, the holdup is simply lost as reduced yield. Exceed Filter Bubble Point – Typically not possible due to system pressure constraints. – May exceed Max Filter Differential Pressure for <0.2 µm membranes. Buffer Flush – Benefits: Removes all residual – Disadvantages Dilutes product (Costly to concentrate or unacceptable) Cost of buffer 7

8. ZenPure Confidential ZenPure’s ZenCap® Zero A patent pending technology, providing ready-to-use full size filter assemblies that have been specially designed for maximizing process efficiency. 8  Optimized Capsule Design Reduces Void Space for Liquid Holdup  Sterile Air Port Allows Access to the Downstream Holdup  Multiple configurations and fittings types come standard

9. ZenPure Confidential Key Features Reduced Void Space Sterile Port Eliminates Downstream Holdup 9 A ZenPure Patent Pending Technology

10. ZenPure Confidential Reducing Holdup to ZERO Compared with other commercially available capsule filters, the ZenCap Zero significantly reduces upstream and downstream holdup, effectively reducing it to ZERO. 10 250-350 ml Saved per 10” Capsule

11. ZenPure Confidential Case Study High Value Diagnostics Cannot dilute final product using buffer flush. 300 ml recovered fluid per run. 250 filtration runs a year. $100 value per ml. Gives $7,500,000 valuable savings per year. 11

12. ZenPure Confidential Complete asepsis: eliminating hose barbs and triclamps. New Filter Capsule Sanitary Connection. 12

13. ZenPure Confidential Complete asepsis: eliminating hose barbs and triclamps. 1000’s of custom single use (SU) bag designs are used in our industry. When over-molded sterile connections are the preferred, the weakest link has been the hose barb to tube filter connection. 13

14. ZenPure Confidential ZERO ingress / ZERO egress filter capsules New Sterile Connection Seamless, gamma stable TPE tube Ready to over-mold Soon: any length, any diameter 14

15. ZenPure Confidential Website: WWW.ZENPURE.COM Email: INFO-US@ZENPURE.COM Mailing address: ZenPure Americas, Inc. 12030 Cadet Court Manassas, VA 20109 Phone: 703-335-9910 Fax: 703-310-6232 Thank You! QUESTIONS?