Relatieve affiniteit voor de alfa en beta receptor.

Slides:

Advertisements

Similar presentations

Biomarkers of Wellness.

Advertisements

Embrace the change with confidence LONG-TERM

Daidzein Genistein Standard YL ML RT FL FR ST Supplementary figure 1.

Beauty Sells, by Beautydesk Sabina Huf. Aging Individualisation Multi-culturalisation Emancipation Critical consumer Less time more wellness Technology.

Menopauze en borst Menopauze oestrogenen oestrogenenstimulatie ter hoogte van borst klierweefsel en steunweefsel en vetweefsel.

And why ?????????????. Breast cancer: -in the Netherlands new cases/year -incidence has doubled since 1940, now 1000/10 6 women/year -30 % of all.

Vitamin D Dr. Pamela Leventis Consultant Rheumatologist

Activation of pro-estrogens from hops by intestinal bacteria Sam Possemiers, Selin Bolca, Veerle Maervoet, Tom Van de Wiele, Arne Heyerick, Denis De Keukeleire.

1 Rôle du microbiote intestinal dans le métabolisme des composés aromatiques polycycliques et hétérocycliques Role of intestinal microbiota in the metabolism.

Laboratory of Microbial Ecology and Technology (LabMET) Research topics and expertise Head : Prof. Dr. ir. Willy Verstraete.

1 Laboratory of Microbial Ecology and Technology (LabMET) Research topics and expertise Tom Van de Wiele, PhD.

Loughborough University Chemistry Department Analytical Chemistry EstradiolGenistein Measurement of Phytoestrogens in Saliva Taghreed Muhammad Fagieh Supervisor/

1 Chemopreventive effects of pro- and prebiotics towards microbial bioactivation of ingested contaminants Inulin and Lactobacillus amylovorus supplemented.

Adding Vitality to life Difference in metabolic phenotype and urinary kinetics of flavonoid metabolites in humans after a black tea and grape juice/red.

Metabolic Signatures in Apoptotic Human Cancer Cell Lines Anna Halama 1, Gabriele Möller 1, and Jerzy Adamski 1,2 Vincent Torrecampo and Tammy Tran.

The Role of Phytoestrogens in Cancer Etiology Susan E. McCann, PhD, RD Department of Epidemiology Division of Cancer Prevention and Population Sciences.

DNA pol a Cyclins E,A B-Myb Estrogen Receptor.

Day 1: TWIN-SHIME inoculation with the same faecal sample. Days 1- 22: Stabilitation period. Days 1t- 11t: Treatment period. Supplementation of Provinols.

METHODS This research study was completed by performing a systematic review of evidence- based literature. The following databases were used: MEDLINE FirstSearch,

Early Enteral Nutrition in the Critically Ill. Objectives To define early enteral nutrition To review the benefits of early enteral nutrition To explain.

- Studiju materiāli / MolekularasBiologijas / MolGen / EN.

PARALLEL SESSION A Auditorium Descartes Selected communications Chairs: Glaucia Maria Pastore - UNICAMP- Campinas - Brazil Peter Hollman - Wageningen University.

WATER o About two-thirds of your body is made up of water. o Water helps keep your body’s temperature stable. o It carries nutrients and oxygen to cells.

INTRODUCTION Analytical characterization of the BBB functionality of the opioid-peptides EM-1, EM-2 and CTAP. S. Van Dorpe 1, N. Pintelon 1, A. Adriaens.

MDA-MB h MDA-MB h a MDA-MB h MDA-MB-468 MDA-MB h MDA-MB h MCF-7 48h MCF-7 72h b c ef g h MDA-MB h clomipramine.

INTRODUCTION Chemical quality control of peptide functionality testing in tissue baths. M. Verbeken 1, S. Van Dorpe 1, R. Lefebvre 2 and B. De Spiegeleer.

MargiAnne Isaia, MD MPH Advanced Nutrition Soy isoflavones and bone Phytochemicals.

Soy: Healthy or Harmful Presented by Megan Hetherington-Rauth, RD.

Omzetting van polluenten in maag-darm systeem

Complementary therapies for menopausal symptoms Complementary therapies for menopausal symptoms Rod Baber.

Updates on the Microbiome: Impacts of current research on nutrition and health. Tiffany Weir, PhD Colorado State University.

Fig. 1 UGT2B15 mRNA levels are stimulated by E2 in a time- and dose-dependent manner in MCF-7 breast cancer cells. A, Time course for E2 treatment. Cells.

Volume 137, Issue 5, Pages (November 2009)

Metabolism and bioavailability of flavanones after administration of normal and functional citrus juices to healthy human volunteers. Medina, S.1; García-Viguera,

Figure 4 Interactions between adipose, the microbiome and kidney

Gut microbiota is responsible for the susceptibility of DSS colitis to chronic stress. Gut microbiota is responsible for the susceptibility of DSS colitis.

Volume 137, Issue 5, Pages (November 2009)

Lycium Barbarum Inhibits Growth of Hormone Responsive Human Breast Cancer Cells by Down-regulating C16α-hydroxylation Pathway of Estradiol Metabolism.

Ticking in Place for the Microbiome to Message Out

Cancer stem cells and their application into targeted therapy for cancer Mol. Bio. Lab Park Ji Won Supervisor ; Dae Youn Hwang.

Supplementary material

Figure 2 Microbiota and osteoimmunology

Lecture Isoflavones.

miRNA expression patterns in stools from healthy subjects.

Cetuximab treatment increases IFNγ receptor 1 expression.

Progesterone receptor nuclear morphology patterns in breast cancer.

Chad M. Trent, Martin J. Blaser Cell Metabolism

Improvement of insulin sensitivity by treatment of the annexin A1 receptor agonist in HFD mice. Improvement of insulin sensitivity by treatment of the.

Figure 4 Local species pools that contribute to the

Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.

IEF 2D PAGE of whole protein extracts from breast apocrine macrocysts

PICRUSt analysis results of predicted functional pathways in the gut microbiota. PICRUSt analysis results of predicted functional pathways in the gut microbiota.

Escape the resistance.

Metabolic labeling of IGFBP-5 by T47D breast carcinoma cells

Correlation between mass spectrometry–based (MS) variance estimates and TMA homogeneity. Correlation between mass spectrometry–based (MS) variance estimates.

Live-cell imaging of transcriptional activation following TSA treatment. Live-cell imaging of transcriptional activation following TSA treatment. (A) Frames.

Fig. 5. Differentiated cells sort to the outer layer, regardless of E- or N-cadherin status.Wildtype and mutant ES cells were distinguished by GFP expression.

Fig. 3. Read-outs of mTORC1 (P-S6(S235/236)) and mTORC2 (P-Akt(S473)) in wtPC12 and PC12-27 cells.(A,B) wtPC12 and PC12-27 cells were treated for 48 hr.

Soy Isoflavones and Their Effect on Breast Cancer

Variation analysis for differences between transcription levels on the duplicated alleles. Variation analysis for differences between transcription levels.

Gut Microbiome Studies

HETE-PEs are increased in leukocytes and platelets from APS patients.

A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety of 24 human cell lines. A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety.

Mir-128 reduction in breast cancer tissues correlates chemotherapy resistance and poor patient survival. Mir-128 reduction in breast cancer tissues correlates.

LRH-1 controls cell proliferation in antiestrogen-sensitive and antiestrogen-resistant breast cancer. LRH-1 controls cell proliferation in antiestrogen-sensitive.

(A) Principal component analysis plot of weighted UniFrac distances of microbiota composition at baseline, week 2 and week 8 and donors. (A) Principal.

Fig. 5 Distributions of cell nuclear area values and internuclear distances in the breast tumor specimens (Figs. 3 and 4), where bin interval = 8 and n.

Gall bladder volumes (mean (SEM)) in the fasting state, during, and after modified sham feeding (MSF) and in response to cholecystokinin (CCK) infusion.

Presentation transcript:

Relatieve affiniteit voor de alfa en beta receptor

Adapted from Boterberg, 2000 E2E2 

GENISTEIN, DAIDZEIN, AND EQUOL AND CELL-CELL ADHESION IN MCF- 7/6 CELLS DMSOEquol Equol + ICI 182,780 Equol + MB2 SLOW AGGREGATION ASSAY

FAST AGGREGATION ASSAY

Het grote probleem variatie in resultaten van studies bij fyto oestrogenen is de grote variatie in opname van deze stoffen.

Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I O-desmethylangolensinEquol 8-prenylnaringenin DaidzeinIsoxanthohumol Enterodiol (END)Enterolactone (ENL) Secoisolariciresinol  Large interindividual variation

Daidzein OHO O OH Dihydrodaidzein (DHD) OHO O O H Equol OHO OH O-desmethylangolensin (O-DMA) OHO O O H H SOY Isoxanthohumol (IX) 8-Prenylnaringenin (8-PN) OMe OH HOP Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009

MIX Daidzein OHO O OH Equol OHO OH IX OMe 8-PN OH ENL SECO R: H GUT MICROBIOTA Daidzein OHO O OH Equol OHO OH IX OMe 8-PN OH ENL SECO R: H GUT MICROBIOTA

Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I Individual phytoestrogens – SHIME run Individual phytoestrogens – SHIME run

Agreement Nr. RT-05/02-FYTOES-1 Ghent, January12 th 2009 WORK PACKAGE I Individual phytoestrogens – SHIME run Individual phytoestrogens – SHIME run Daidzein metabolism (Decroos et al. (2006) J Nutr 136, ) Daidzein metabolism (Decroos et al. (2006) J Nutr 136, ) Weak equol-producing inoculum + EPC4 Weak equol-producing inoculum + EPC4 Standard SHIME feed µM daidzein aglycon eq. Standard SHIME feed µM daidzein aglycon eq. IX metabolism (Possemiers et al. (2006) J Nutr 136, ; Possemiers et al. (2008) J Nutr 138, ) IX metabolism (Possemiers et al. (2006) J Nutr 136, ; Possemiers et al. (2008) J Nutr 138, ) Strong 8-PN-producing inoculum Strong 8-PN-producing inoculum Weak 8-PN-producing inoculum + E. limosum Weak 8-PN-producing inoculum + E. limosum Standard SHIME feed + 70 µM IX Standard SHIME feed + 70 µM IX  Stable EQUOL production in colon transversum  Stable 8-PN production in colon transversum

BREAST TISSUE HOMOGENATE

MATERIAL & METHODS - BREAST FFQ, BMI, age, antibiotic use,… bloodspot urine Breath methane R WASHOUT TREATMENT GC-FID AESTHETIC BREAST SURGERY Breast biopsies Enzymatic deconjugation Extraction HPLC-UV-MS Enzymatic deconjugation Extraction HPLC-UV-MS Bio-activity biodistribution concentration molecular form